Identifikasi Bakteri Pseudomonas sp. dan Shigella sp. Dari Feses Bekantan (Nasalis larvatus), Primata Endemik Kalimantan

Abstract

Bakteri Shigella sp. dan Pseudomonas sp. merupakan bakteri patogen penyebab penyakit pencernaan paling umum pada hewan primata dan non human primate. Penelitian ini bertujuan mengisolasi dan mengidentifikasi bakteri patogen Pseudomonas sp. dan Shigella sp. yang terdapat pada feses Bekantan (Nasalis larvatus). Sampel yang digunakan adalah delapan sampel feses bekantan koleksi dari Laboratorium Bakteriologi, Divisi Mikrobiologi Medik, Sekolah Kedokteran Hewan dan Biomedis, Institut Pertanian Bogor. Isolasi dilakukan dengan metode cawan gores pada media MacConkey Agar (MCA) dan identifikasi dengan uji biokimia dan uji gula-gula mengacu pada Cowan and Steel’s Manual for the Identification of Medical Bacteria.Hasil isolasi dan identifikasi diperoleh tiga isolat yang memiliki karakteristik sesuai Shigella sp. dan satu isolat bakteri Pseudomonas sp.

Shigella sp. and Pseudomonas sp. are the most common pathogenic bacteria that cause gastrointestinal diseases in primates and non-human primates. This study aims to isolate and identify the pathogenic bacteria, Pseudomonas sp. and Shigella sp., found in the feces of proboscis monkeys (Nasalis larvatus). A total of eight proboscis monkey feces samples were collected from the Bacteriology Laboratory, Medical Microbiology Division, School of Veterinary Medicine and Biomedical Medicine, Bogor Agricultural University. Bacteria in proboscis monkey feces were isolated by streak plate methode on MacConkey Agar (MCA) media and identification by biochemical test and sugar test, according to Cowan and Steel’s Manual for the Identification of Medical Bacteria. The test results confirmed that these bacteria accurately presented three isolates matching characteristics to Shigella sp. and one isolate of Pseudomonas sp.