Penyembuhan Luka Sayat pada Tikus Putih (Rattus norvegicus) dengan Pemberian Lendir Bekicot (Achatina fulica)

Abstract

Lendir bekicot (Achatina fulica) memiliki kandungan yang dapat mempercepat penyembuhan luka dan aktivitas antibakteri, sehingga sering digunakan sebagai obat alternatif. Tujuan penelitian ini adalah menganalisis dan membandingkan peran lendir bekicot yang diberi perlakuan stres dan tidak terhadap proses serta kecepatan penyembuhan luka sayat pada tikus putih secara topikal. Penelitian ini menggunakan 24 ekor tikus yang diberi empat luka sayat pada bagian punggung, luka diberi sediaan kontrol (negatif dan positif) dan lendir bekicot (stres/S dan tanpa perlakuan stres/TS) selama tujuh hari. Persentase sisa panjang luka dihitung pada hari ke-3, 5, 7, dilanjutkan pengamatan histopatologi. Metode Minimum Inhibitory Concentration (MIC) dilakukan untuk menguji aktivitas antibakteri lendir bekicot. Proses penyembuhan luka dengan lendir TS lebih cepat, ditunjukkan dengan persentase sisa panjang luka pada perlakuan S dan TS masing-masing sebesar 85,84% dan 80,34% (H-3), 75,64% dan 73,89% (H-5), 53,27% dan 52,16% (H-7). Uji MIC menunjukkan lendir S dan TS memberi daya hambat pertumbuhan bakteri Escherichia coli dan Pseudomonas aeruginosa.

Snail mucus (Achatina fulica) has ingredients that can accelerate wound healing and antibacterial activity, so it is often used as an alternative medicine. The purpose of this study was to analyze and compare the role of snail mucus treated with stress and not on the process and speed of wound healing in white rats topically. This study used 24 rats that were given four cuts on the back, the wounds were treated with control preparations (negative and positive) and snail mucus (stress/S and no stress/TS treatment) for seven days. The percentage of remaining wound length was calculated on days 3, 5, 7, followed by histopathological observation. The Minimum Inhibitory Concentration (MIC) method was performed to test the antibacterial activity of snail mucus. The wound healing process with TS mucus was faster, indicated by the percentage of remaining wound length in the S and TS treatments of 85.84% and 80.34% (H-3), 75.64% and 73.89% (H-5), 53.27% and 52.16% (H-7), respectively. MIC test showed that S and TS mucus inhibited the growth of bacteria such as Escherichia coli and Pseudomonas aeruginosa.