Evaluation of Influenza Antibody Titers in Serum and Egg Yolk of Layers Vaccinated with Influenza Vaccines.
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Date
2023-07-21Author
Pathmanathan, Divheyaa
Poetri, Okti Nadia
Amrozi
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Show full item recordAbstract
Influenza belongs to the Orthomyxoviridae family which causes upper and
lower respiratory tract infection. The aim of this study was to evaluate influenza-A
specific antibody titers from serum and egg yolk of hens vaccinated with
quadrivalent influenza vaccines. Three Hy-line Brown layers were vaccinated with
quadrivalent influenza type A and B inactivated vaccines. Vaccination was carried
out three times at a dose of 0.25 ml/chicken via the intramuscular route. The first
vaccination was carried out at 24 weeks of age, then boosters were performed at 28
and 32 weeks of age. Serum and egg yolk were collected every week for 20 weeks
since the first vaccination. Influenza A H3N2 and H1N1 antibody titers were
determined using hemagglutination-inhibition (HI) test and agar-gel-precipitation
(AGP) test. Influenza-A H3N2 and H1N1 specific antibodies were detected in both
serum and egg yolk from the first to twenty weeks after the initial vaccination.
Highest HI geometric mean titer (GMT) of H1N1 antibody reached 26.67 HIU at
nine weeks post first vaccination (p.f.v), while H3N2 antibody reached 26 HIU at
six weeks p.f.v. Our result showed that influenza antibodies can be produced in
layers using quadrivalent vaccines and layer chickens are able to produce equivalent
antibody levels for each influenza subtype.