Identification and Pattern of Antibiotic Resistance of Pseudomonas spp. Isolated from Orangutan (Pongo Pygmaeus) at Bogor Indonesia Safari Park

Abstract

Pseudomonas spp. is a lactose-negative gram-negative bacteria that contributes to several infections including air sacculitis in orangutans due to its having the largest air sac compared to most nonhuman primates. This research was designed to study and analyze the identification and the pattern of antibiotic resistance of Pseudomonas spp. Identification of Pseudomonas includes KOH 3% test, oxidase test, TSIA test, urea test, IMViC test Indole, Methyl-Red, Voges-Proskauer, and Citrate) and carbohydrate fermentation test. Pattern of antibiotic resistance includes five different types of antibiotics from different antibiotic classes which are ampicillin from β- lactam, gentamicin from aminoglycoside, tetracycline from tetracycline, enrofloxacin from fluoroquinolone, and chloramphenicol from phenicol class. As the results, identification of Pseudomonas spp. showed three suspected isolates of Pseudomonas spp. Patterns of antibiotic resistance was carried out by using Kirby-Baurer method which uses agar disk diffusion resulted in two of the isolates that were mostly similar in pattern, resistant to only ampicillin and are sensitive towards tetracycline, enrofloxacin, chloramphenicol, and gentamicin meanwhile the other one of the isolate differ, resistant to ampicillin, tetracycline, enrofloxacin, and chloramphenicol and is sensitive towards gentamicin. In conclusion, feces samples of orangutans revealed three suspected Pseudomonas spp. and showed resistance on ampicillin and sensitive to gentamicin. The study of antibiotic resistance patterns is crucial for future antibiotic selection.