Identifikasi dan Pola Resistansi Klebsiella sp. yang Diisolasi dari Feses Orang Utan Kalimantan (Pongo pygmaeus) di Taman Safari Indonesia, Bogor

Abstract

Orang utan merupakan satu-satunya kera besar yang masih bertahan di Wilayah Asia Tenggara. Saat ini, populasi orang utan di habitatnya terus mengalami penurunan. Diperkirakan selama 60 tahun terakhir populasi orang utan berkurang sebanyak 50%. Data mikrobiologi yang membahas resistansi bakteri Klebsiella sp. terhadap antibiotik pada feses orang utan kalimantan (Pongo pygmaeus) belum banyak dilakukan. Penelitian ini bertujuan untuk mengidentifikasi keberadaan bakteri Klebsiella sp. serta mengetahui pola resistansinya terhadap beberapa antibiotik pada feses P. pygmaeus. Metode penelitian terdiri dari isolasi, identifikasi, dan uji resistansi antibiotik. Hasil isolasi dan identifikasi ditemukan sebanyak tiga isolat (37,5%) yang positif bakteri Klebsiella sp. Berdasarkan uji sensitivitas, isolat Klebsiella sp. yang berasal dari feses P.pygmaeus sehat di Taman Safari Indonesia, Bogor, berhasil didapatkan dua pola resistansi berbeda yang memberikan gambaran adanya sensitivitas dan resistansi terhadap beberapa antibiotik. Klebsiella sp. telah mengalami resistan terhadap antibiotik golongan β-laktam dan masih sensitif terhadap antibiotik golongan aminoglikosida, tetrasiklin, dan fluorokuinolon.

Orangutans are the only great apes that still survive in the Southeast Asian Region. Currently, the orangutans population in their habitat continues to decline. It is estimated that over the last 60 years, the orang utan population has decreased by 50%. Microbiological data discussing the resistance of Klebsiella sp. against antibiotics in the feces of the bornean orangutans (Pongo pygmaeus) has not been widely carried out. This study aims to identify the presence of Klebsiella sp. as well as know the pattern of resistance to several antibiotics in the feces of bornean orangutans. The research method consisted of isolation, identification, and antibiotic resistance testing. The results of isolation and identification found that three isolates (37.5%) were positive for Klebsiella sp. Based on the sensitivity test, Klebsiella sp. isolates which come from the feces of healthy P.pygmaeus in Taman Safari Indonesia, Bogor, two different patterns of resistance have been obtained which provide an overview of sensitivity and resistance to several antibiotics. Klebsiella sp. has experienced resistance to β-lactam class antibiotics and is still sensitive to aminoglycoside, tetracycline, and fluoroquinolone antibiotics.