Host Range Study of Papaya ringspot virusIsolates from Bogor and Medan

Abstract

Papaya ringspot disease was first reported in Nanggroe Aceh Darussalam, Indonesia, in 2012. Since then, PRSV spread rapidly in papaya growing areas in Java, Sumatra and Bali. PRSV infection causes immediate yield loss due to the symptoms of ringed spots on the fruit which reduces the quality and quality of the fruit. This study aimed to evaluate the incidence, severity and symptoms of ring spot disease caused by two PRSV isolates, i.e. isolates Bogor and Medan on some varieties of papaya (“Callina”, “Miba”, “Sukma”, and “California) and plants from the family Cucurbitaceae, i.e. cucumber (Cucumis sativus), watermelon (Citrullus lanatus), bitter gourd (Momordica charantia), and melon (Cucumis melo). The source of virus isolates was taken from the collection of Plant Virology Laboratory, IPB. Virus inoculation was conducted mechanically using sap of virus inoculum. Disease symptoms was observed for 5 weeks and PRSV infection was confirmed by DAS-ELISA. Typical symptoms of PRSV infection was observed on papaya var. Callina, California, and Sukma, but did not appear on papaya var. Miba and all Cucurbitaceae. The types of symptoms that appeared on the leaves include mosaic, chlorotic spots, vein clearing, blisters, wrinkles, crunching, thickening of the lamina, and leaf malformations (“shoestring”). Disease incidence in var. Callina, California, and Sukma ranges from 33% to 100% while disease severity ranges from 6% to 86.11%. Infection of PRSV-Medan tends to cause higher severity than PRSV-Bogor