| dc.contributor.advisor | Darusman, Huda Shalahudin | |
| dc.contributor.advisor | Karja, Ni Wayan Kurniani | |
| dc.contributor.author | Tsabita, Aqila | |
| dc.date.accessioned | 2023-07-10T06:45:12Z | |
| dc.date.available | 2023-07-10T06:45:12Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/121243 | |
| dc.description.abstract | Diagnosis dini dari penyakit Alzheimer hingga kini masih sulit untuk dilakukan, sehingga penting dilakukan penelitian lebih lanjut untuk menemukan biomarker yang dapat digunakan untuk mendeteksi penyakit ini secara dini. Proliferating Cell Nuclear Antigen (PCNA) dikenal sebagai marka proliferasi, yang berpotensi mendeteksi neurogenesis di otak. Penelitian ini bertujuan untuk mengidentifikasi ekspresi gen PCNA di otak sebagai marka penyakit Alzheimer pada Macaca fascicularis. Macaca fascicularis digunakan karena kemiripannya dengan manusia dalam kompleksitas perilaku, kemampuan kognitif yang tinggi dan pembentukan karakteristik patologis penyakit Alzheimer di otak. Penelitian ini menggunakan otak regio hipokampus dan korteks dari 7 ekor monyet. Monyet sebelumnya telah dibagi menjadi kelompok usia tua dan dewasa. Deteksi ekspresi gen PCNA dilakukan dengan metode RT-qPCR. Hasil penelitian menunjukkan ekspresi gen cenderung lebih tinggi pada monyet kelompok dewasa dan di regio hippocampus, meskipun berdasarkan analisis statistik tidak menunjukkan perbedaan yang signifikan. | id |
| dc.description.abstract | Early diagnosis of Alzheimer's is still difficult to do, thus it is important to carry out further research to find biomarkers that can be used to detect early Alzheimer's disease. Proliferating Cell Nuclear Antigen (PCNA) is known as a proliferation marker, which has the potential to detect neurogenesis in the brain. This study aimed to identify PCNA gene expression in the brain as a marker of Alzheimer’s disease in Macaca fascicularis. Macaca fascicularis was used in this study because of their similarities with humans in terms of their behavioral complexity, as well as high cognitive abilities and the formation of a pathological characteristic of Alzheimer's disease in the brain. This study used the brains of 7 monkeys in the hippocampus and cortical regions. Monkeys previously have been divided into old and adult age groups. The detection of PCNA gene expression was done using RT-qPCR method. The results showed the gene expression tended to be higher in the adult group and the hippocampus region, although based on statistical analysis showed no significant differences. | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Identifikasi Ekspresi Gen PCNA sebagai Marka Penyakit Alzheimer pada Otak Monyet Ekor Panjang (Macaca fascicularis) | id |
| dc.title.alternative | Identification of PCNA Gene Expression as the Marker of Alzheimer Disease in Cynomolgus Monkey (Macaca fascicularis) | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | Alzheimer's disease | id |
| dc.subject.keyword | Macaca fascicularis | id |
| dc.subject.keyword | Neurogenesis | id |
| dc.subject.keyword | PCNA | id |
| dc.subject.keyword | RT-qPCR | id |
