Uji Hipoglikemik Ekstrak Industri Gabungan Daun Undis (Cajanus cajan) dan Jahe (Zingiber officinale) pada Tikus
Date
2023Author
Putri, Anisya Saeila
Wresdiyati, Tutik
Santoso, Koekoeh
Metadata
Show full item recordAbstract
Diabetes melitus merupakan salah satu penyakit yang sangat mematikan di
dunia yang ditandai oleh tingginya kadar glukosa darah. Penelitian ini bertujuan
menguji efek hipoglikemik ekstrak industri gabungan daun undis (Cajanus cajan)
dan jahe (Zingiber officinale) pada tikus model hiperglikemia galur Sprague
Dawley. Ekstraksi dilakukan di PT Insular Multi Natural dengan pelarut alkohol
70% dan metode maserasi. Efek hipoglikemik dilakukan dengan metode Oral
Glucose Tolerance Test (OGTT). Kondisi hiperglikemia pada tikus didapatkan
dengan induksi larutan sukrosa 90%. Sejumlah 30 ekor tikus jantan Sprague
Dawley telah digunakan pada penelitian ini, yang dibagi menjadi enam kelompok
perlakuan, yaitu kelompok kontrol negatif/KN (akuades), kontrol positif/KP
(sukrosa), kontrol obat/KO (glibenclamide + sukrosa), serta ekstrak (ekstrak dosis
100, 200, dan 400 mg/kg BB + sukrosa). Hasil penelitian menunjukkan bahwa
ekstrak gabungan daun undis dan jahe memiliki efek hipoglikemik. Berdasarkan
profil perubahan kadar glukosa darah dan luas area di bawah kurva, ekstrak dosis
200 mg/kg BB menunjukkan efek hipoglikemik dosis optimum. Diabetes mellitus is a very deadly disease in the world which is characterized
by high blood glucose levels. This study aims to examine the hypoglycemic effect
of industrial extract of combination Cajanus cajan leaves and ginger (Zingiber
officinale) in hyperglycemia-model Sprague Dawley rats. Extraction was carried
out at PT Insular Multi Natural with 70% alcohol solvent and maceration method.
The hypoglycemic effect was carried out using the Oral Glucose Tolerance Test
(OGTT) method. Hyperglycemia conditions in rats were obtained by induction of
90% sucrose solution. A total of 30 male Sprague Dawley rats were used in this
study, which were divided into six treatment groups, namely negative control/KN
(aquades), positive control/KP (sucrose), drug control/KO (glibenclamide +
sucrose), extract (doses of 100, 200, and 400 mg/kg BW + sucrose). The results
showed that the extract of combination C. cajan leaves and ginger had a
hypoglycemic effect. Based on the profile of changes in blood glucose levels and
the area under the curve, the extract at a dose of 200 mg/kg BW showed the
optimum dose of hypoglycemic effect.