| dc.contributor.advisor | Darusman, Huda Shalahudin | |
| dc.contributor.advisor | Retnani, Elok Budi | |
| dc.contributor.author | Ningrum, Emilna Mega | |
| dc.date.accessioned | 2022-11-28T23:32:37Z | |
| dc.date.available | 2022-11-28T23:32:37Z | |
| dc.date.issued | 2022-11-25 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/115376 | |
| dc.description.abstract | Penyakit Alzheimer adalah penyakit neurodegeneratif yang merusak otak
secara bertahap dan merupakan bagian dari demensia. Penyakit demensia
Alzheimer disebabkan oleh berbagai faktor sehingga sampai saat ini belum
diketahui dengan pasti terapi yang tepat. Pembuatan kit sebagai alat diagnostik
untuk menunjang pengembangan penapisan terhadap penyakit Alzheimer
merupakan upaya strategis yang dapat dilakukan. Penelitian ini bertujuan untuk
melakukan kajian reliabilitas kit deteksi amiloid yang dikembangkan menjadi uji
alternatif penapisan penyakit Alzheimer dibandingkan dengan kit komersial.
Penapisan berbasis pendeteksian dengan teknik Enzyme-Linked Immunosorbent
Assay (ELISA) dapat bekerja secara efektif dan akurat dalam mengoptimalkan
bahan antibodi spesifik atau antibodi monoklonal terhadap peptida Aβ42.
Berdasarkan hasil dan interpretasi dari nilai batas deteksi, koefisien inter dan intra-
assay, kit ELISA yang dikembangkan secara in house memiliki kemampuan deteksi
Aβ42 yang menyamai Kit ELISA komersial yang telah tersedia (MyBioSource
MBS288507).
Kata kunci: Aβ42, ELISA, penapisan, penyakit Alzheimer, reliabilitas kit | id |
| dc.description.abstract | Alzheimer’s Disease is a progressive neurodegenerative disease that
damages the brain and is part of dementia. Alzheimer’s dementia is caused by
various factors that until now the proper therapy is uncertain. Kit production as a
diagnostic tool to support the development of screening for Alzheimer’s disease is
a strategic effort. Screening based on detection with Enzyme-Linked
Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a
specific antibody material or monoclonal antibody to the Aβ42 peptide. This study
aimed to research the reliability of the amyloid detection kit which was developed
as an alternative screening test for Alzheimer’s disease compared to commercially
available kits. Based on the results and interpretation of limits of detection, inter
and intra-assay coefficients, the in-house ELISA kit has comparable Aβ42 detection
to the commercially available ELISA kit (MyBioSource MBS288507).
Keywords: Aβ42, Alzheimer’s disease, ELISA, reliability kit, screening | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Studi Reliabilitas Kit Deteksi Amiloid Sebagai Penapisan Penyakit Alzheimer | id |
| dc.title.alternative | Reliability Study of Amyloid Kit for Detection of Alzheimer's Disease | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | ELISA | id |
| dc.subject.keyword | Reliabilitas kit | id |
| dc.subject.keyword | Macaca fascicularis | id |
| dc.subject.keyword | Penyakit Alzheimer | id |
| dc.subject.keyword | Penapisan | id |
| dc.subject.keyword | Aβ42 | id |
