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      Identifikasi dan Total Bakteri Fermentatif Asam Laktat pada Feses Primata

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      Date
      7-07-22
      Author
      Putri, Faraditha Nakita
      Safika
      Afiff, Usamah
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      Abstract
      Bakteri asam laktat (BAL) bermanfaat bagi kesehatan, terutama peranannya sebagai probiotik dan antimikroba dalam pengobatan penyakit saluran pencernaan pada primata. Penelitian ini bertujuan untuk mengidentifikasi serta menghitung total koloni bakteri asam laktat pada feses lima jenis primata yaitu orang utan, owaungko hitam, owa ungko coklat, siamang, dan lutung yang dikoleksi dari Kebun Binatang Bukittinggi. Sampel dilakukan pengenceran 10-1 hingga ke 10-7 dandiinokulasikan ke dalam media selektif MRSA (de Man Rogosa Sharpe Agar) dengan metode pour plate. Koloni bakteri asam laktat yang tumbuh pada media diamati dan dihitung total koloninya. Koloni terpilih diisolasi untuk selanjutnya diidentifikasi menggunakan beberapa uji biokimia yaitu uji katalase, uji oksidase, uji Sulfide Indol Motility, uji Methyl Red-Voges Proskauer, dan uji fermentasi karbohidrat. Penelitian berhasil mengisolasi bakteri dari genus Lactobacillus, Leuconostoc, dan Weissella. Hasil penelitian menunjukkan orang utan menghasilkan total bakteri asam laktat tertinggi yaitu sebesar 3,1 x 108 CFU/ml.
       
      Lactic acid bacteria (LAB) are beneficial for health, especially their role as probiotics and antimicrobials in the treatment of digestive tract diseases in primates. This research aims to identify and count the total colonies of lactic acid bacteria in the feces of five primate species, namely orangutans, black gibbons, brown gibbons, siamang, and langurs collected from the Bukittinggi Zoo. Samples were diluted 10- 1 to 10-7 and inoculated into MRSA (de Man Rogosa Sharpe Agar) selective media using the pour plate method. Colonies of lactic acid bacteria growing on the media were observed and the total colonies were counted. Selected colonies were isolated for further identification using several biochemical tests, namely catalase test, oxidase test, Sulfide Indole Motility test, Methyl Red-Voges Proskauer test, and the carbohydrate fermentation test. The results of the research succeeded in isolating lactic acid bacteria from the genera Lactobacillus, Leuconostoc, and Weissella. Orangutans produced the highest total lactic acid bacteria, which was 3.1 x 108 CFU/ml.
       
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      http://repository.ipb.ac.id/handle/123456789/112752
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      • UT - Animal Disease and Veterinary Health [945]

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