Nematoda Pucuk Putih Padi Aphelenchoides besseyi Christie di Sumatera Barat.

Abstract

Aphelenchoides besseyi is an important pest that could reduce rice production. West Sumatera. This study aims to explore the presence of A. besseyi, to detect and identify seed-borne A. besseyi nematode in West Sumatera utilizing morphology and morphometric characters. Samples of rice seeds used are local varieties of rice and hybrid varieties from Agam District, Lima Puluh Kota District, and Solok District. Methods include nematode extraction from seeds, population counting, morphological and morphometric identification, and preparation of slide preparate. The results of this study showed that A. besseyi have infected Junjuang, Sokan, Kuriak, Pulau Sarundang, Sarundang Baru, Batang Pasaman, Pulau Bendang, Pulau Mundam, Bendang Baru, 42 C, Pulut Merah, CK, Mundam Pasaman, Damasraya, Koritiang, Ale-Ale, Hitam Putih, Sijunjung, Junjuang, Sokan, Marujang Bujang, Batang Piaman, Anak Daro, Cisokan, PB 42, and 42 C with number of nematode populations from 1.56-125.44 / 10 g of seed. The morphological characters of A. besseyi found were a vermiform body, slender, smooth body annulation, set off lips, a spear-like stylet with an enlarged knob at the base, a large and oval-shaped median bulb, clear esophageal ring, overlapping esophagus, vulva located at 60 -75% of the body, the posterior part of the body of the male nematode is curved up to 90 °, the spicules are rose thorns, and 2-3 star shaped mucros. Morphometric characteristics with total body length (L) 362.54-672.70 μm, stilet length (S) 9.80-14.01 μm, body diameter (D) 10.41-16.86 μm, tail length (T) 23.81-39.66 μm, vulva distance from anterior divided by total body length (V) 54.48-75.97%, L / D (A) 33.86-63.58 μm, L / distance of the esophageal base from the anterior (B) 5.18-9.97 μm, L / T (C) 9.84-26.04 μm.