Biodelignifikasi Tongkol Jagung Menggunakan Fungi Pelapuk Putih (White Rot Fungi)

Abstract

Corncob has big potency to be converted become simple sugar such as glucose and xylose which turn can be used as raw material for various products for instance sorbitol and xylitol. Biodelignification is a process to remove lignin and to make fibre free from this subtance by using microorganism like mould, bacteria or enzyme. Delignification need to be done because lignin is main barrier at cellulose hydrolysis process. Some types white rot fungi often applied for delignification are Schizophyllum commune, Phanerochaete chrysosporium and isolate Pleurotus EB9. The aims of this research is to obtain the best type of white rot fungi and to get optimum concentration of mycelial and concentration of glucose which is added and optimum incubation time of chosen best fungi. The result of preliminary research that corncob delignification by fungi Phanerochaete chrysosporium had highest decreasing of lignin content that was 26.07% and degradation of dry weight that is 28.22%. Therefore, Phanerochaete chrysosporium was chosen to be used in main research. Result of main research showed that optimum condition for delignification of corncob by Phanerochaete chrysosporium was incubation time 24.25 days, quantity of mycelial 1.89 ml/10 g substrate and number of glucoses added 0.23 g/10 g substrate. From this condition the value of lignin rate ratio to holocellulose was 0.210689 g/g.