Hidrolisis Pati Palma Menggunakan Pullulanase dan β-Amilase

Abstract

Palm trees are unexplored carbohydrate sources that wiidely spread in Indonesia. Palm starches are still under utilized since lacked information about their pysico-chemical properties. Hydrolysis of these starches are the right application without concerning their characteristics. This research investigated the starches from five types of palm trees, namely Metroxylon sago (commersial sago), Metroxylon sp. (sagu rumbia), Arenga pinnata, Arenga microcarpa (sagu baruk), and Caryota mitis; and the hydrolysis of palm starches by using degrading enzymes, i.e. pullulanase and β- amylase. The result showed that palm starches are normal starch which contain17-21% of amylose or 83-79% of amylopectin which differed in branch-chain lengths, and caused typical products from each type of palm. Pullulanase as debranching enzymes, cleaveged the starch to produce DP 13.9- 20.8 of Short-Chains Amylose (SCA). The starch contained 22.2-38.8% of β-limit dextrins as the residue of β-amylolysis. Simultaneous hydrolysis by using β-amylase and pullulanase mainly produced maltose for all starch with DP of 1.7-2.9.