Kajian penanganan limbah hasil delignifikasi dan hidrolisis tandan kosong kelapa sawit (TKKS) menggunakan kultur mikrobial campuran (mixed culture) cairan rumen sapi

Abstract

During EFBO's delignification and acidic hydrolysis process, not all of lignocellulotic materials can be converted into monomer source. Handling of the by-product was carried out using mixed culture of cattle's rumen juice in anaerobic condition. The best result of the handling on the ratio of delignification medium by-product:hydrolysis:buffer solvent was 1:1:1 {w/v/v). Degradation of the liquid mixrure on a 3 titre bioreactor during eleven days decreased COD percentage of 69,68% and and biogas product of 5120 mililitres. Kinetic parameters of rumen microorganism on the liquid mixture resulted that saw rate of product forming on substrate (Y p/s - 0.14 ml% NDF), biomass growth rate on substrate (Y x/s - 0.60 colony/ml% NDF), specific maximum growth rate (fm = 0.81/day), gas volume at time the substrate fully degradated (Vf - 5 190 60 ml), maximum gas forming rate {was 0.15 m!% NDF/day), inflection time of gas forming/half time when gas forming was on the lag phase <1 — 09 day).