dc.description.abstract | During EFBO's delignification and acidic hydrolysis process, not all of lignocellulotic materials can be converted into monomer source. Handling of the by-product was carried out using mixed culture of cattle's rumen juice in anaerobic condition. The best result of the handling on the ratio of delignification medium by-product:hydrolysis:buffer solvent was 1:1:1 {w/v/v). Degradation of the liquid mixrure on a 3 titre bioreactor during eleven days decreased COD percentage of 69,68% and and biogas product of 5120 mililitres. Kinetic parameters of rumen microorganism on the liquid mixture resulted that saw rate of product forming on substrate (Y p/s - 0.14 ml% NDF), biomass growth rate on substrate (Y x/s - 0.60 colony/ml% NDF), specific maximum growth rate (fm = 0.81/day), gas volume at time the substrate fully degradated (Vf - 5 190 60 ml), maximum gas forming rate {was 0.15 m!% NDF/day), inflection time of gas forming/half time when gas forming was on the lag phase <1 — 09 day). | en |