Potensi Senyawa Aktif Ekstrak Etanol Buah Okra Merah dengan Pemupukan Kasgot BSF sebagai Inhibitor α-Glukosidase secara In silico

Abstract

Kandungan senyawa aktif buah okra merah diketahui memiliki aktivitas inhibisi α-Glukosidase tetapi belum diketahui pengaruh penggunaan pupuk kasgot BSF (Black soldier fly) terhadap kandungan senyawa aktif buah okra merah sebagai inhibitor α-glukosidase. Penelitian bertujuan menganalisis pengaruh penggunaan pupuk kasgot terhadap kandungan senyawa buah okra merah dan potensinya sebagai inhibitor α-glukosidase secara in silico. Tanaman okra merah ditanam dengan 3 perlakuan pupuk, yaitu tanpa pupuk (P1), pupuk kandang (P2), dan pupuk kasgot BSF (P3). Buah hasil panen diekstraksi dengan metode maserasi menggunakan pelarut etanol 70% dan ekstrak dianalisis dengan LC-MS/MS. Potensinya diprediksi, sebagai inhibitor α-glukosidase dengan kode PDB 2QMJ melalui studi in silico. Rangkaian studi in silico menghasilkan 5 senyawa buah okra merah potensial. Penggunaan pupuk kandang dan pupuk kasgot BSF pada tanaman okra merah sama-sama menghasilkan senyawa yang baik sebagai inhibitor α-glukosidase. Senyawa terbaik buah okra merah P2 dan P3, scortechinone B memiliki nilai ΔGikatan sebesar -9,2 kkal/mol, berikatan pada triad katalitik α-glukosidase, Tyr299, Asp443, dan Asp542.

The content of active compounds in red okra fruit is known to have α-glucosidase inhibitory activity but the effect of using BSF (Black soldier fly) kasgot fertilizer on the active compound content of red okra fruit as an α-glucosidase inhibitor is still unknown. The study aimed to analyze the effect of kasgot fertilizer on the compound content of red okra fruit, and its potential as an α-glucosidase inhibitor by in silico method. Red okra plants were planted with 3 fertilizer treatments, such as without fertilizer (P1), manure (P2), and BSF kasgot fertilizer (P3). The harvested fruit then extracted by maceration method with 70% ethanol solvent and the extract was analyzed using LC-MS/MS, and its potential as an α-glucosidase inhibitor with PDB code 2QMJ was predicted through an in silico study. The whole in silico study resulted in 5 potential red okra fruit compounds. The conclusion of this study is that the use of manure and BSF kasgot fertilizer on red okra plants both produce good compounds as α-glucosidase inhibitors. The best compounds of red okra fruit P2 and P3, scortechinone B having ΔGbind as much as -9,2 kkal/mol, binding at triad catalytic of α-glucosidase, Tyr299, Asp443, and Asp542.