Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/78037
Title: | Staphylococcal Enterotoxin A Gene-Carrying Staphylococcus aureus Isolated from Foods and Its Control by Crude Alkaloid from Papaya Leaves |
Authors: | Lita Handayani Didah Nur Faridah Harsi D. Kusumaningrum |
Issue Date: | 2014 |
Publisher: | Journal of Food Protection |
Citation: | Journal of Food Protection. Vol 77; No 11; 2014; Hal 1992-1997 |
Abstract: | Staphylococcus aureus is a known pathogen causing intoxication by producing enterotoxins in food. Staphylococcal enterotoxin A is one of the enterotoxins commonly implicated in staphylococcal food poisoning. The ability of crude alkaloid extract from papaya leaves to inhibit the growth of S. aureus and staphylococcal enterotoxin A synthesis was investigated. Staphylococcal enterotoxin A gene-carrying S. aureus was isolated from raw milk and ready-to-eat food s. Crude alkaloid was extracted from ground, dried papaya leaves using ultrasonic-assisted extraction, and a MIC of the alkaloid was determincd by the broth macrodilution method. Furthermore, S. aureus isolate was exposed to the crude alkaloid extract at one- and twofold MIC, and the expression of sea was subsequently analyzed using a quantitative reverse transcription real-time PCR. Ten isolates of S. aureus were obtained, and nine of those isolates were sea carriers. The yield of crude alkaloid extract was 0.48 to 1.82% per dry weight of papaya leaves. A MIC of crude alkaloid to S. aureus was 0.25 mg/ml. After exposure to the alkaloid at 0.25 and 0.5 mg/ml for 2 h, a significant increase in cycle threshold values of sea was observed, The sea was expressed 29 and 41 times less when S. aureus was exposed to crude alkaloid at one- and twofold MTC respectively. This study revealed that crude alkaloid of papaya leaves could control staphylococcal enterotoxin A gene-carrying S. aureus by suppressing the expression of sea, in addition to the ability to inhibit the growth of S. aureus. The expression of sea was successfully quantified. |
URI: | http://repository.ipb.ac.id/handle/123456789/78037 |
ISSN: | 1944-9097 |
Appears in Collections: | Food Science and Technology |
Files in This Item:
File | Size | Format | |
---|---|---|---|
Staphylococcal-Journal of Protection-Didah N F (gabung).pdf | 12.37 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.