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DC Field | Value | Language |
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dc.contributor.author | Lita Handayani | - |
dc.contributor.author | Didah Nur Faridah | - |
dc.contributor.author | Harsi D. Kusumaningrum | - |
dc.date.accessioned | 2016-01-30T07:10:44Z | - |
dc.date.available | 2016-01-30T07:10:44Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Journal of Food Protection. Vol 77; No 11; 2014; Hal 1992-1997 | id |
dc.identifier.issn | 1944-9097 | - |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/78037 | - |
dc.description.abstract | Staphylococcus aureus is a known pathogen causing intoxication by producing enterotoxins in food. Staphylococcal enterotoxin A is one of the enterotoxins commonly implicated in staphylococcal food poisoning. The ability of crude alkaloid extract from papaya leaves to inhibit the growth of S. aureus and staphylococcal enterotoxin A synthesis was investigated. Staphylococcal enterotoxin A gene-carrying S. aureus was isolated from raw milk and ready-to-eat food s. Crude alkaloid was extracted from ground, dried papaya leaves using ultrasonic-assisted extraction, and a MIC of the alkaloid was determincd by the broth macrodilution method. Furthermore, S. aureus isolate was exposed to the crude alkaloid extract at one- and twofold MIC, and the expression of sea was subsequently analyzed using a quantitative reverse transcription real-time PCR. Ten isolates of S. aureus were obtained, and nine of those isolates were sea carriers. The yield of crude alkaloid extract was 0.48 to 1.82% per dry weight of papaya leaves. A MIC of crude alkaloid to S. aureus was 0.25 mg/ml. After exposure to the alkaloid at 0.25 and 0.5 mg/ml for 2 h, a significant increase in cycle threshold values of sea was observed, The sea was expressed 29 and 41 times less when S. aureus was exposed to crude alkaloid at one- and twofold MTC respectively. This study revealed that crude alkaloid of papaya leaves could control staphylococcal enterotoxin A gene-carrying S. aureus by suppressing the expression of sea, in addition to the ability to inhibit the growth of S. aureus. The expression of sea was successfully quantified. | id |
dc.language.iso | en | id |
dc.publisher | Journal of Food Protection | id |
dc.title | Staphylococcal Enterotoxin A Gene-Carrying Staphylococcus aureus Isolated from Foods and Its Control by Crude Alkaloid from Papaya Leaves | id |
dc.type | Article | id |
dc.subject.keyword | Staphylococcus aureus | id |
Appears in Collections: | Food Science and Technology |
Files in This Item:
File | Size | Format | |
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Staphylococcal-Journal of Protection-Didah N F (gabung).pdf | 12.37 MB | Adobe PDF | View/Open |
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