Identifikasi Keragaman Gen Insulin-like Growth Factor-1 pada Kambing Peranakan Etawah di BPTU KDI-HPT Pelaihari Kalimantan Selatan

Abstract

Etawah grade goats meat productions can be improved through the efforts of livestock genetic enhancement based on the exploration of potential genes that supposedly strong influence in growth trait. Insulin-like growth factor-1 (IGF-1) gene is known to be associated with the milk production and growth traits. The objective of this research was to identify the polymorphism of IGF-1 gene in Etawah grade goats from BPTU KDI-HPT Pelaihari South Kalimantan. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method used to detect the genotype of IGF-1 gene. Polymorphism in 5’-flanking region of IGF-1 gene was studied in 115 Etawah grade goats blood samples. A primer designed specifically was used to amplify a fragment DNA of IGF-1 locus along 249 bp. Restriction with SnaBI revealed one allele B was cut at one restriction site in fragment 249 bp. The frequencies for allele B was 1.00, resulting monomorphic allele. Based on Ge et al. (2001) and Siadkowska et al. (2006) researchs, allele B was associated on weight and body size.