Viabilitas Lactobacillus acidophilus dan Bifidobacterium bifidum Terenkapsulasi dan Mutu Sensori Yogurt Tepung Pisang Sinbiotik selama Penyimpanan Dingin

Abstract

Two types of probiotic i.e. Lactobacillus acidophilus and Bifidobacterium bifidum were encapsulated using alginate (3%) and palm sago starch (20%). Yogurt product were then stored at 4 – 10 oC for 8 weeks. Viabilities of the probiotics, pH and titratable acidity were examined in week 0,2,4,5,6,7 and 8. Viabilities of L. acidophilus either free or encapsulated and the pH value did not change during 8 weeks storage, while total titratable acidity increased. L. acidophilus demonstrated better survival compared to B. bifidum. Cell numbers of B. bifidum either free or encapsulated decreased significantly at the range of 1 – 2 log CFU/g during 8 weeks storage. Encapsulation of B. bifidum in palm sago starch protected the cells better than alginate. The cell numbers of B. bifidum encapsulated in alginate decreased approximately 2 log CFU/g at week 8, while ii cells encapsulated in palm sago starch only decreased of 1 log CFU/g. Sensory evaluation of the yogurt samples were performed at week 0, 4 and 5. The consistency of yogurt did not affected during 5 weeks storage while other sensory properties including color, texture, and aroma were slightly change. Yogurt containing L. acidophilus either free or encapsulated in palm sago starch were still accepted at week 5, while yogurt with alginate encapsulated L. acidophilus was not accepted at week 4.