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Title: | Evaluasi keragaman genetik gen hormon pertumbuhan (GH) pada sapi pesisir Sumatera Barat menggunakan penciri PCR-RFLP |
Authors: | Jakaria Solihin, Dedy Duryadi Noor, R.R. Tappa, B. Martojo, H. |
Keywords: | Pesisir cattle growth hormone gene PCR-RFLP polymorphism |
Issue Date: | 2007 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol 30 No. 1; |
Abstract: | A total of 134 Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of the GH Mspl and GH AluI Pesisir cattle were determined. The GH Mspl gene frequencies for the C and T allele were 0.209 and 0.791 respectively, while GH AluI gene frequencies for the L and V allele were 0.992 and 0.008 respectively. The chi-square analysis indicated that this population is not in Hardy-Weinberg Equilibrium status. Expected heterozygosis value (He) for GH Mspl and GH AluI were 0.3306±0.0266 and 0.0149±0.0073 respectively. The PCR-RFLP AluI marker. This finding showed that GH Mspl T allele was favorable as a GH marker for Bos indicus breeds. |
URI: | http://repository.ipb.ac.id/handle/123456789/59011 |
ISSN: | 0126-0472 |
Appears in Collections: | Media Peternakan |
Files in This Item:
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media peternakan.pdf | 365.72 kB | Adobe PDF | View/Open |
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