Pengaruh Suhu Dan Waktu Penyeduhan Teh Hijau (Camellia Sinensis) serta Proses Pencernaan In Vitro terhadap Aktivitas Inhibisi Lipase

Abstract

Obesity will lead to many other degenerative diseases. One of the alternative solutions to prevent obesity is by inhibiting the digestion of lipids. Green tea extract has anti-lipase activity. This research aims to determine the effect of time (5, 10, and 15 minutes) and temperature (70, 85, and 100˚C) of green tea brewing and in vitro digestion on lipase inhibition. The research method is divided into two stages, green tea brewing and in vitro gastrointestinal pH simulation. Green tea extract is analyzed for anti-lipase activity, total phenol, and condensed tannin level. The results showed that the temperature and time of brewing influence the level of lipase inhibition, total phenol, and condensed tannin of green tea extract. The total phenols and condensed tannin level have a strong correlation with the lipase inhibition. The green tea initial extract (before in vitro digestion) in 85˚C for 5 min and 100˚C temperature in all the time showed the highest inhibition activity. But, after in vitro digestion, only green tea which brewed in 100˚C for 5 minutes and 10 minutes which had the best inhibition of lipase. There are no difference in significant level for inhibition activity between green tea brewed in 100˚C brewing temperature for 5 minutes and 10 minutes.