Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/57162
Title: | Flavonoid Buah Andaliman (Zanthoxylum acanthopodium DC) sebagai Antioksidan dan Inhibitor Enzim α-glukosidase Flavonoid of Zanthoxylum achanthopodium Fruit as an Antioxidant and α-Glucosidase Inhibitors |
Authors: | Suparto, Irma Herawati Batubara, Irmanida Gultom, Sofyan |
Keywords: | Zanthoxylum acanthopodium DC antidiabetic antioxidant inhibitor α-glukosidase flavonoid |
Issue Date: | 2011 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Diabetes Mellitus could be caused by the presence of free radicals which damaged the pancreas cell membrane. Previous research showed that extract of andaliman (Zanthoxylum acantophodium DC.) fruit possessed activity as antioxidant. However, there has been no scientific report concerning its activities as antidiabetic. The main objective of this research was to evaluate the in vitro mechanism of Z. acantophodium fruit extract as antioxidant and α-glucosidase inhibitor. Andaliman macerated by four solvents, namely n-hexane, ethyl acetate, methanol and water, as solvent for extraction. Antioxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis -3- ethylbenzathiazolin -6-sulfonic acid (ABTS) method, and inhibition of enzyme α-glucosidase using substrate p-nitrofeyl-α-D-glucopyranoside. Results of this research showed that methanol extract had the highest activities, both as antioxidant and α-glucosidase inhibitor. This extract was separated by column chromatography yielded six fractions (A-F). The most active fraction as α-glucosidase inhibitor was fraction C and for antioxidant was fraction E. These two fractions were further separated using preparative thin layer chromatography. The best antioxidants with DPPH method was fraction E2 (IC50 137.46 ppm), for ABTS method was crude methanol extract (IC50 30.04 ppm), while for α-glucosidase inhibitors was the fraction C (IC50 16 ppm). Based on the results of data analysis by Infrared and UV-Vis, showed that the main active compound contained in fraction C2 and E2 were the of aurones and flavanones of flavanoid group. |
URI: | http://repository.ipb.ac.id/handle/123456789/57162 |
Appears in Collections: | MT - Mathematics and Natural Science |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
2011sgu.pdf Restricted Access | full text | 1.71 MB | Adobe PDF | View/Open |
BAB I PENDAHULUAN.pdf Restricted Access | BAB I | 394.74 kB | Adobe PDF | View/Open |
BAB II TINJAUAN PUSTAKA.pdf Restricted Access | BAB II | 560.79 kB | Adobe PDF | View/Open |
BAB III METODOLOGI PENELITIAN.pdf Restricted Access | BAB III | 445.21 kB | Adobe PDF | View/Open |
BAB IV HAASIL DAN PEMBAHASAN.pdf Restricted Access | BAB IV | 689.29 kB | Adobe PDF | View/Open |
BAB V SIMPULAN DAN SARAN.pdf Restricted Access | BAB V | 394.41 kB | Adobe PDF | View/Open |
COVER.pdf Restricted Access | Cover | 378.62 kB | Adobe PDF | View/Open |
DAFTAR PUSTAKA.pdf Restricted Access | Daftar Pustaka | 407.52 kB | Adobe PDF | View/Open |
LAMPIRAN.pdf Restricted Access | Lampiran | 779.16 kB | Adobe PDF | View/Open |
RINGKASAN.pdf Restricted Access | Ringkasan | 407.01 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.