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Title: | Kinetika Inhibisi Flavonoid Sidaguri (Sida rhombi/alia L.) Terhadap Enzim Xantin Oksidase |
Authors: | Darusman, Latifah Kosim Pradono, Dyah Iswantini Hidayat, Ramdan |
Keywords: | Bogor Agricultural University (IPB) sidaguri Javonoid xanthine oxidase inhibition |
Issue Date: | 2007 |
Abstract: | Sidaguri is one of the traditional medicinal plants that has a potency as antigout. It has been reported that flavonoid from sidaguri could inhibit activity of x~hine oxidase enzyme (causes gout) up to 55,29% (Iswantini & Darusman, 200~ The aim of this research is to investigate the type of sidaguri's flavonoid crud~ extract kinetic inhibition and the effects of purification on inhibitory poterWY that have never been studied. The result of this research showed that sidagWi's extraction has yielded 11,85% flavonoid crude extract (LCso 501,23 ppm)~ith inhibitory effect from 47,71 % until 70,71% (100-800 ppm). Generally, the eSract has a good potential as gout medicine although it was tested in low conc~tration from the extract's toxicity rate. The kinetic inhibition of 200 ppm crudeDextract caused the increment ofKM (1,15 mM or increase 132%) from 0,87 roM i> 2,02 roM and without changed of V max. Based on that, the type of VI inhibinon is competitive inhibition (Webb 1963). Purification yielded 11 fracti~s and the highest activity came from fraction 4 (inhibitory effect of 79,09!'o). The purification has caused low increase of potent inhibitory effect. Fract~n 3 with 100 ppm has a bit higher inhibitory effect (51,58%) compare to the cI!oe extract at same concentration (47,71%) or increase 8,11%. In general the poten1l:linhibitory effect from purification result has lower strength compare to the 100 Ifm crude extract. GC-MS analysis from fraction 4 showed that there were 39 or..gruric compounds with BM estimation of 152-454 glmol. Based on FT-IR and GC-MS analysis, strengthened with phytochemical test we estimate that the potential compound with xanthine oxidase inhibitory activity is flavonoid compound. |
URI: | http://repository.ipb.ac.id/handle/123456789/55213 |
Appears in Collections: | MT - Mathematics and Natural Science |
Files in This Item:
File | Description | Size | Format | |
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2007rhi.pdf Restricted Access | full text | 3.03 MB | Adobe PDF | View/Open |
Abstract.pdf Restricted Access | Abstract | 356.28 kB | Adobe PDF | View/Open |
BAB I Pendahuluan.pdf Restricted Access | BAB I | 366.69 kB | Adobe PDF | View/Open |
BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 1.14 MB | Adobe PDF | View/Open |
BAB III Bahan dan Metode.pdf Restricted Access | BAB III | 483.61 kB | Adobe PDF | View/Open |
BAB IV Hasil dan Pembahasan.pdf Restricted Access | BAB IV | 734.54 kB | Adobe PDF | View/Open |
BAB V Kesimpulan dan Saran.pdf Restricted Access | BAB V | 346.25 kB | Adobe PDF | View/Open |
Cover.pdf Restricted Access | Cover | 284.7 kB | Adobe PDF | View/Open |
Daftar Pustaka.pdf Restricted Access | Daftar Pustaka | 416.15 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 958.5 kB | Adobe PDF | View/Open |
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