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dc.contributor.advisorDarusman, Latifah Kosim
dc.contributor.advisorPradono, Dyah Iswantini
dc.contributor.authorHidayat, Ramdan
dc.date.accessioned2012-06-26T04:42:18Z
dc.date.available2012-06-26T04:42:18Z
dc.date.issued2007
dc.identifier.urihttp://repository.ipb.ac.id/handle/123456789/55213
dc.description.abstractSidaguri is one of the traditional medicinal plants that has a potency as antigout. It has been reported that flavonoid from sidaguri could inhibit activity of x~hine oxidase enzyme (causes gout) up to 55,29% (Iswantini & Darusman, 200~ The aim of this research is to investigate the type of sidaguri's flavonoid crud~ extract kinetic inhibition and the effects of purification on inhibitory poterWY that have never been studied. The result of this research showed that sidagWi's extraction has yielded 11,85% flavonoid crude extract (LCso 501,23 ppm)~ith inhibitory effect from 47,71 % until 70,71% (100-800 ppm). Generally, the eSract has a good potential as gout medicine although it was tested in low conc~tration from the extract's toxicity rate. The kinetic inhibition of 200 ppm crudeDextract caused the increment ofKM (1,15 mM or increase 132%) from 0,87 roM i> 2,02 roM and without changed of V max. Based on that, the type of VI inhibinon is competitive inhibition (Webb 1963). Purification yielded 11 fracti~s and the highest activity came from fraction 4 (inhibitory effect of 79,09!'o). The purification has caused low increase of potent inhibitory effect. Fract~n 3 with 100 ppm has a bit higher inhibitory effect (51,58%) compare to the cI!oe extract at same concentration (47,71%) or increase 8,11%. In general the poten1l:linhibitory effect from purification result has lower strength compare to the 100 Ifm crude extract. GC-MS analysis from fraction 4 showed that there were 39 or..gruric compounds with BM estimation of 152-454 glmol. Based on FT-IR and GC-MS analysis, strengthened with phytochemical test we estimate that the potential compound with xanthine oxidase inhibitory activity is flavonoid compound.en
dc.subjectBogor Agricultural University (IPB)en
dc.subjectsidagurien
dc.subjectJavonoiden
dc.subjectxanthine oxidaseen
dc.subjectinhibitionen
dc.titleKinetika Inhibisi Flavonoid Sidaguri (Sida rhombi/alia L.) Terhadap Enzim Xantin Oksidaseen
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