Lama pemberian pakan mengandung tepung meniran Phyllanthus niruri dan bawang putih Allium sativum untuk pencegahan infeksi bakteri Aeromonas hydrophila pada ikan lele dumbo Clarias sp.

Abstract

Motile Aeromonad Septicaemia (MAS) caused by Aeromonas hydrophila induced serious epidemics of disease in freshwater fish one catfish Clarias sp. This research purposed was to determine optimum durations of the application feed contains of mixed powders Pyllanthus niruri and Allium sativum to preventing MAS. Experimental design was Complete Random Design that consist 3 treatmens and 3 replications. This research tested 3 different durations of 7 application of P. niruri and A. sativum, namely A (feed with P. niruri and A. sativum powder application during 21 days and infected with A. hydrophila 0,1 ml), B (feed with P. niruri and A. sativum powder application during 14 days and infected with A. hydrophila 0,1 ml), C (feed with P. niruri and A. sativum powder application during 7 days and infected with A. hydrophila 0,1 ml), and control, namely K- (without P. niruri and A. sativum powder application and injected with PBS 0,1 ml) and K+ (without P. niruri and A. sativum powder application and infected with A. hydrophila 0,1 ml). The treatments was given for 21 days before challenging test, at 22th day test in vivo carried out by injecting A. hydrophila (108 CFU/ml) into the fish by intramuscular and observed for 10 days. The highest value of survival rate that consist in treatment K⁻ was 100 ± 0.00%, treatment A was 93.3 ± 11.55%, and treatment B was 73.33 ± 30.55%. While treatment K+ and C have the same survival rate that is, 26.67 ± 11.55%. The application P. niruri and A. sativum powder during 21 days was optimum for preventing MAS in catfish.