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http://repository.ipb.ac.id/handle/123456789/47480
Title: | Viabilitas embrio mencit (Mus musculus albinus) setelah kriopreservasi ganda dengan metode vitrifikasi pada tahap pembelahan dan blastosis |
Authors: | Widarto, Tri Heru Boediono, Arief Saftiany, Riska |
Keywords: | Bogor Agricultural University (IPB) |
Issue Date: | 2011 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Embryo freezing technique nowadays has developed rapidly in accordance with the success of embryo engineering technology. The objective of this research was to test the endurance and the survival rate of the mice embryos after double cryopreservation of 8 cells and blastocysts stages by vitrification method. The media used for this study were: a) equilibrium media (Phosphate Buffer Saline, (PBS)) + 20% serum + 10% ethylene glycol), b) vitrification media (PBS + 20% serum + 0.5 M sucrose + 15% ethylene glycol + 15% DMSO), c) rehydration media (PBS + 20% serum + different concentration of sucrose solution (0.5M, 0.25M, 0.1M sucrose)), and d) in vitro culture media (G2, Vitrolife, Swedia). The results showed that the viability of mice embryos developed to hatched blastocysts after double vitrification (42.11%) were similar (P>0.05) to embryo viability after single vitrification of 8 cells or blastocysts stages. However, it was different (P<0.05) with the control (94.87%) of the embryo reached hatched blastocysts stages. |
URI: | http://repository.ipb.ac.id/handle/123456789/47480 |
Appears in Collections: | UT - Biology |
Files in This Item:
File | Description | Size | Format | |
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G11rsa.pdf Restricted Access | fulltext | 2.13 MB | Adobe PDF | View/Open |
Abstract_ G11rsa.pdf Restricted Access | Abstract | 297.08 kB | Adobe PDF | View/Open |
BAB I Pendahuluan_ G11rsa.pdf Restricted Access | BAB I | 551.49 kB | Adobe PDF | View/Open |
BAB II Bahan dan Metode_ G11rsa.pdf Restricted Access | BAB II | 553.49 kB | Adobe PDF | View/Open |
BAB III Hasil_ G11rsa.pdf Restricted Access | BAB III | 1.84 MB | Adobe PDF | View/Open |
BAB IV Simpulan_ G11rsa.pdf Restricted Access | BAB IV | 305.01 kB | Adobe PDF | View/Open |
Cover_ G11rsa.pdf Restricted Access | Cover | 300.07 kB | Adobe PDF | View/Open |
Daftar Pustaka_G11rsa.pdf Restricted Access | Daftar Pustaka | 299.51 kB | Adobe PDF | View/Open |
G11rsa.pdf Restricted Access | Full Text | 2.14 MB | Adobe PDF | View/Open |
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