Viabilitas embrio mencit (Mus musculus albinus) setelah kriopreservasi ganda dengan metode vitrifikasi pada tahap pembelahan dan blastosis

Abstract

Embryo freezing technique nowadays has developed rapidly in accordance with the success of embryo engineering technology. The objective of this research was to test the endurance and the survival rate of the mice embryos after double cryopreservation of 8 cells and blastocysts stages by vitrification method. The media used for this study were: a) equilibrium media (Phosphate Buffer Saline, (PBS)) + 20% serum + 10% ethylene glycol), b) vitrification media (PBS + 20% serum + 0.5 M sucrose + 15% ethylene glycol + 15% DMSO), c) rehydration media (PBS + 20% serum + different concentration of sucrose solution (0.5M, 0.25M, 0.1M sucrose)), and d) in vitro culture media (G2, Vitrolife, Swedia). The results showed that the viability of mice embryos developed to hatched blastocysts after double vitrification (42.11%) were similar (P>0.05) to embryo viability after single vitrification of 8 cells or blastocysts stages. However, it was different (P<0.05) with the control (94.87%) of the embryo reached hatched blastocysts stages.