Morphology and Biometry of Spermatozoa of the Anoa (Bubalus sp) Stained Using William's and Eosin-Nigrosin

Abstract

Anoa is Indonesia endemic fauna that included into Appendix I by CITES. Anoa breeding efforts have not succeed, due to their agressivity, soliter, wildness and monogamous. The aim of this experiment was to observe morphology and biometry of anoa’s sperm stained using William’s (W) and eosin-nigrosin (EN). Semen was collected from two male anoas by electroejaculator. Slides was made for morphology and biometry observation of ejaculate. The results showed that abnormal sperm morphology of the mature anoa (10 years) was 32.27%-35.00%, meanwhile the young one (3 years) was 24.03%–31.89%. In mature anoa, measurement for head width, and midpiece and tail length of sperm were not signfi cantly diff erent between W and EN staining, but signifi cantly diff erent (P<0.05) for head and total length. In the young anoa, sperm biometry for head width was not signifi canly diff erent between W and EN staining. In both W and EN staining, biometry for head width and total length were not signifi cantly diff erent between mature and young anoas, but signifi cantly diff erent for head, midpiece and tail length. According to sperm morphology, semen from the young anoa was slightly better than those of the mature anoa. Staining method and age could aff ect to biometry of the anoa’s sperm.