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Title: | Bioenergetic Analysis of FLAG Tagged-Subunit 8 of Saccharomyces cerevisiae Mitochondrial ATP Synthase |
Other Titles: | Microbiology Indonesia Vol.4 No.3 Tahun 2010 |
Authors: | Artika, I Made |
Issue Date: | 2010 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol.4;No.3 |
Abstract: | The majority of cellular energy in the form of adenosine triphosphate (ATP) is synthesized by the F F -ATP synthase. The yeast mitochondrial F F -ATPsynthase is a multisubunit complex that contains at least 17 different subunits grouped into F and F sectors. Subunit 8 of yeast mitochondrialATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to the C-terminus of subunit 8 and its variants has facilitated elucidation of subunit 8's membrane topology. In order to analyze its detailed structure and function, a set of strains expressing FLAG tagged-subunit 8 and its variants were subjected to bioenergetic analysis at cellular and mitochondrial levels. Results obtained showed that the hydrophobic character of the central hydrophobic domain of subunit 8 is essential for functional coupling between F and F sectors, hence for mitochondrialATPsynthase function. |
URI: | http://repository.ipb.ac.id/handle/123456789/43002 |
ISSN: | 1978-3477 |
Appears in Collections: | Journal of Microbiology Indonesia |
Files in This Item:
File | Description | Size | Format | |
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I Made Artika.pdf | e-Journal | 75.94 kB | Adobe PDF | View/Open |
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