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Title: | Expression of Simian Retrovirus Type D Serotype 2 Envelope in Insect Cell Using Baculovirus Expression Vector System |
Other Titles: | Microbiology Indonesia Vol.3 No.2 Tahun 2009 |
Authors: | Saepuloh, Uus Iskandriati, Diah Sadikin, Mohamad Pamungkas, Joko |
Issue Date: | 2009 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol.3;No.2 |
Abstract: | Simian retrovirus type-D (SRV) is a causative agent of simian acquired immunodeficiency syndrome in Asian macaques, and can serve as a viral model in understanding of retrovirus infection because of some similarities to human AIDS pathogenesis. Study of infection and pathogenesis of SRV in macaques could be a strategy of vaccine and antiviral development for preventive and therapeutic purposes. We expressed the SRV-2 envelope gene using baculovirus expression vector system and transfected it to Spodoptera frugiferda insect cell line for SRV-2 recombinant protein production. Analysis using PCR and sequencing technique of recombinant in the passage-3 viral stock indicated the occurrence of recombination between SRV-2 envelope and baculovirus genome. Purification using immobilized metal ion affinity chromatography Ni2+-NTA to recombinant protein could minimize the presence non-specific proteins. The SDS-PAGE analysis showed a specific protein for SRV-2 gp70 envelope. Western blot analysis of this purified protein indicated a specific reaction with anti-SRV-2 antibody positive of Macaca fascicularis serum shown as SRV-2 gp70 envelope band. |
URI: | http://repository.ipb.ac.id/handle/123456789/42958 |
ISSN: | 1978-3477 |
Appears in Collections: | Journal of Microbiology Indonesia |
Files in This Item:
File | Description | Size | Format | |
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Uus Saepuloh.pdf | e-Journal | 174.95 kB | Adobe PDF | View/Open |
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