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Title: | Free-Living Ice-Nucleating Active Bacteria from High Mountain Lake Habitats |
Other Titles: | Microbiology Indonesia Vol.2 No.3 Tahun 2008 |
Authors: | Yuhana, Munti Hanselmann, Kurt |
Issue Date: | 2008 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol.2;No.3 |
Abstract: | We collected the culturable heterotrophic bacteria from oligotrophic high mountain lake habitats and tested their capability to induce ice formation. Direct plating was carried out using low-nutrient medium at a temperature of between 3 and 4°C. As many as 84 isolates were recovered from glacial ice and natural biofilm growing on granite rocks surface. Six out of 84 isolates were capable of expressing the ice-nucleation phenotype. After autoclaving the cell suspension at 121°C for 15 min, isolate J78 was still able to retain the ability for ice formation. Heat-stable ice nuclei produced by ice-nucleating active bacteria have potential applications in biotechnology. Characterization of INA bacteria was performed employing live-dead Gram staining and molecular methods. Universal primers for Bacteria (S-D-Bact-0008-b-S-20 and S-D-Bact-1524-a-A-18) were used for PCR to amplify almost the full length of the 16S rRNA genes of selected INA isolates. Restriction fragment length polymorphism analysis resulted in 2 unique patterns, as represented by J43 and J83, respectively. Based on DNA sequencing of 16S rRNA gene, isolate J43 (GeneBank accession no. AJ864852) was closely related to Pseudomonas mephitica (99.2% sequence similarity) and Janthinobacterium lividum (99% similarity), whereas isolate J83 (GeneBank accession no. AJ864859) showed 100% sequence identity to Pseudomonas fluorescens. |
URI: | http://repository.ipb.ac.id/handle/123456789/42922 |
ISSN: | 1978-3477 |
Appears in Collections: | Journal of Microbiology Indonesia |
Files in This Item:
File | Description | Size | Format | |
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Munti Yuhana.pdf | e-Journal | 132.64 kB | Adobe PDF | View/Open |
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