Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/42897
Title: Identity and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Tomato in Indonesia
Other Titles: Microbiology Indonesia Vol.2 No.1 Tahun 2008
Authors: Santoso, Tri Joko
Hidayat, Sri Hendrastuti
Duriat, Ati Sri
Herman, Muhammad
Sudarsono
Issue Date: 2008
Publisher: IPB (Bogor Agricultural University)
Series/Report no.: Vol.2;No.1
Abstract: Infection of tomato by Begomovirus is known to cause serious disease and yield losses. Samples of tomato plants showing typical symptoms of begomovirus infection were collected from eight locations in Java and Sumatra. Amplification of a putative AV1 gene was performed using AV1 specific primers for Geminivirus, total nucleic acid isolated from tomato samples exhibiting leaf curl disease as the template, and the PCR technique. Direct sequencing of PCR product was carried out, followed by nucleotide and predicted amino acid sequence analysis using the BLAST program. Positive results were obtained, the PCR amplification proved that diseased tomato samples collected from eight locations in Java and Sumatra were infected with Begomovirus. When nucleic acid and amino acid sequences of the eight isolates were compared to other begomovirus’s sequences present in the GenBank it was found that the isolates determined in this research were Indonesian isolates of AYVV. Further phylogenetic analysis of eight Begomovirus isolates identified in this study indicated they belonged into two different clades. Results of this research also suggest that the existence of Begomovirus genetic diversity in various regions in Indonesia needs further investigation. Moreover, the prevalence of distinct Begomovirus species or isolates also need investigation.
URI: http://repository.ipb.ac.id/handle/123456789/42897
ISSN: 1978-3477
Appears in Collections:Journal of Microbiology Indonesia

Files in This Item:
File Description SizeFormat 
Tri Joko Santoso.pdfe-Journal119.39 kBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.