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Title: | Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization |
Other Titles: | Microbiology Indonesia Vol.1 No.2 Tahun 2007 |
Authors: | Yuratmoko, Deri Mubarik, Nisa Rachmania Meryandini, Anja |
Issue Date: | 2007 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol.1;No.2 |
Abstract: | Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 ºC, whereas that of the keratinase was at pH 6.5 and 70 oC. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 ºC whereas the keratinase was at pH 8.5 and 80 oC. |
URI: | http://repository.ipb.ac.id/handle/123456789/42867 |
ISSN: | 1978-3477 |
Appears in Collections: | Journal of Microbiology Indonesia |
Files in This Item:
File | Description | Size | Format | |
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Deri Yuratmoko.pdf | e-Journal | 242.8 kB | Adobe PDF | ![]() View/Open |
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