Fed-Batch Fermentation Processes to Produce dextranase fro of Streptococcus sp.B7

Abstract

The research aimed to develop fermentation technique on dextranase production from the bacterial isolate of Streptococcus sp. B7 using fed-batch Fermentation system. The production was done in two litre Fermentor with flow rate of medium addition at 19 mI/hr after 24 hours until 72 hours of incubation process. The variable conditions were pH of 7 and 8 and agitation speeds of 300 and 500 rpm. Maximum production was achieved at 500 rpm and p11 8 that produced enzyme activity of 920 U/mI. The best result of some kinetic parameters were as follows : production p = 920 U/mI, productivity Qv = 16.43 U/mllhr, dextranase specific activity 17.68 U/mg protein, yield of product over cell Yp/x = 696.47 U/g cell and specific productivity q, = 12.45 U/g celllhr.