Peranan Fruktosa, Rafinosa, Trehalosa Pada Kriopreservasi Semen Kuda

Abstract

The objective of this study was to evaluate the effectiveness of carbohydrates supplementation on the stallion semen cryopreservation. Semen was collected from 3 stallions using artificial vagina twice a week. Collected semen was evaluated macroand microscopically. Semen showed >60% progressive motility was then divided into 3 tubes and diluted with skim milk 1 : 1, centrifuged at 1 006 g for 10 minutes. Supematan was removed and each pellet rediluted either with skim milk extender supplemented with 50 mM trehalose (ST); 50 mM raffinose (SR) or 100 mM fructose (SF) with the concentration of sperm were 200xlOhml-I. Extended semen packed into minitub 0.3 ml and equilibrates at 4 OC for 2 hours, freezes in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196 "C for further evaluation. After 24 hours, the semen was thawed at 37 "C for 30 second. The results of this experiment indicated that trehalose supplementation in skim milk was found to significantly improve the percentage of sperm motility (Pq0.05) for stallion 1 and 3 compared to rafinose and fructose. But in all stallions, trehalose and fructose were superior compared to raffinose.