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http://repository.ipb.ac.id/handle/123456789/41266
Title: | Characterization of chitosanase of bacillus licheniformis mb-2 isolated from manado hot spring water |
Authors: | Chasanah, Ekowati |
Issue Date: | 2004 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Chitosanase (EC 3.2.1.132) is an important enzyme in the production of chitosan oligomers. This research dealt with chitosanase of Indonesian origin, which was produced by chitinolytic bacteria isolated from Manado hot spring water, referred to as B. licheniformis MB-2. Specifically, the aims of the research were: (1) to produce and purify chitosanase from the bacteria, (2) analyze biochemical characteristics of the enzyme, (3) classify the enzyme based on biochemicd and preliminary bioinfmatics molecular study. The result showed that the optimum media for producing the enzyme were 0.24% chitosan, 0.25% casiton, 1% MgS04, 1.4% K2HP04, 0.02% CaC12.2H20, 0.002% FeS04.7H20. Enzyme harvested at the third day had an activity of 0.7 U/mL, while that harvested at the seventh day showed an activity of 0.8 U/mL |
URI: | http://repository.ipb.ac.id/handle/123456789/41266 |
Appears in Collections: | DT - Agriculture Technology |
Files in This Item:
File | Description | Size | Format | |
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Cover_2005ech.pdf Restricted Access | Cover | 468.02 kB | Adobe PDF | View/Open |
Bab I_2005ech.pdf Restricted Access | Bab I | 317.01 kB | Adobe PDF | View/Open |
Bab II_2005ech.pdf Restricted Access | Bab II | 470.58 kB | Adobe PDF | View/Open |
Bab III_2005ech.pdf Restricted Access | Bab III | 540.85 kB | Adobe PDF | View/Open |
Bab IV_2005ech.pdf Restricted Access | Bab IV | 4.35 MB | Adobe PDF | View/Open |
Bab V_2005ech.pdf Restricted Access | Bab V | 344.51 kB | Adobe PDF | View/Open |
Daftar Pustaka_2005ech.pdf Restricted Access | Daftar Pustaka | 10.3 MB | Adobe PDF | View/Open |
2004ech.pdf Restricted Access | Full Text | 19.12 MB | Adobe PDF | View/Open |
2004ech_abstract.pdf Restricted Access | abstract | 63.35 kB | Adobe PDF | View/Open |
2004ech_abstract.ps Restricted Access | abstract | 415.94 kB | Postscript | View/Open |
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