Characterization of chitosanase of bacillus licheniformis mb-2 isolated from manado hot spring water

Abstract

Chitosanase (EC 3.2.1.132) is an important enzyme in the production of chitosan oligomers. This research dealt with chitosanase of Indonesian origin, which was produced by chitinolytic bacteria isolated from Manado hot spring water, referred to as B. licheniformis MB-2. Specifically, the aims of the research were: (1) to produce and purify chitosanase from the bacteria, (2) analyze biochemical characteristics of the enzyme, (3) classify the enzyme based on biochemicd and preliminary bioinfmatics molecular study. The result showed that the optimum media for producing the enzyme were 0.24% chitosan, 0.25% casiton, 1% MgS04, 1.4% K2HP04, 0.02% CaC12.2H20, 0.002% FeS04.7H20. Enzyme harvested at the third day had an activity of 0.7 U/mL, while that harvested at the seventh day showed an activity of 0.8 U/mL