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http://repository.ipb.ac.id/handle/123456789/27889
Title: | Metabolism of Myo-Inositol and Growth in Various Sugars of Suspension cultured Tobacco Cells |
Authors: | Harran, S. D. B. Dickinson |
Issue Date: | 1978 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Tobacco (Nicotiana tabacum L.) cells were cultured in a liquid medium which contained sucrose as a source of carbon and energy. Various cell-wall constituents and wall precursors (Larabinose, D-xylose, D-galactose, D-mannose, D-glucuronate, myo-inositol) were added to cells growing in this medium to by-pass possible rate-limiting steps in the relevant metabolic pathways. None of these compounds stimulated growth as measured by increase in fresh weight; myo-inositol did cause a slight increase and L-arabinose a decrease in dry weight accumulation compared to controls grown on sucrose only. Although myo-inositol was not needed for rapid growth, tracer level amounts of [2-3H]myo-inositol were rapidly absorbed and metabolized. Label was incorporated into the uronide and pentose residues of cell walls and exocellular polysaccharide. |
URI: | http://repository.ipb.ac.id/handle/123456789/27889 |
ISSN: | IPB (Bogor Agricultural University) |
Appears in Collections: | Faculty of Agriculture |
Files in This Item:
File | Description | Size | Format | |
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33.doc | Publications | 25.5 kB | Microsoft Word | View/Open |
33.pdf | Publications | 15.27 kB | Adobe PDF | ![]() View/Open |
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