Kapasitas Antioksidan dan Inhibisi Lipase Pankreas Hasil Kombinasi Kayu Secang dan Daun Serai Wangi secara In Vitro

Abstract

Obesitas merupakan kondisi akumulasi lemak berlebih dalam jaringan yang menyebabkan gangguan kesehatan. Orlistat, obat sintetik antiobesitas, memiliki efek samping dalam jangka panjang. Kayu secang (Caesalpinia sappan) dan daun serai wangi (Cymbopogon nardus) berpotensi sebagai antiobesitas. Penelitian bertujuan menentukan kadar fenolik, flavonoid total, kapasitas antioksidan, serta aktivitas inhibisi lipase pankreas kombinasi kayu secang dan daun serai wangi. Sampel diekstraksi dengan etanol 70%, dikombinasikan, diuji total fenolik, flavonoid, kapasitas antioksidan (DPPH, FRAP), uji inhibisi lipase, serta analisis data. Hasil penelitian menunjukkan total fenolik, flavonoid, dan antioksidan DPPH serta FRAP pada kayu secang lebih tinggi dibandingkan daun serai wangi yaitu berturut-turut sebesar 33.75 mg GAE/g ekstrak, 16.17 mg QE/g ekstrak, 762.58 mg TE/g ekstrak, 1221.57 mg TE/g ekstrak. Kombinasi rasio secang:serai 1:1, 1:2, 2:1, 1:3, dan 3:1 cenderung menunjukkan efek aditif. Inhibisi lipase oleh secang tunggal (70.96%) lebih baik dibandingkan dengan seluruh kombinasi, namun tidak lebih baik dari orlistat (76.83%).

Obesity is a accumulation of excess fat in tissues that causes health disorders. Orlistat, a synthetic anti-obesity drug, has side effects when used long-term. Secang wood (Caesalpinia sappan) and lemongrass leaves (Cymbopogon nardus) have potential as anti-obesity agents. This study aims to determine the total phenolic, total flavonoids, antioxidant capacity, and pancreatic lipase inhibitory activity of combined of secang wood and lemongrass leaves. Samples were extracted with 70% ethanol, combined in various ratios, and tested for total phenolics, flavonoids, antioxidant capacity (DPPH, FRAP), lipase inhibition, and data analysis. The results showed that the total phenolic, flavonoids, and antioxidant capacity by DPPH and FRAP in secang wood were higher than those in lemongrass leaves, with values of 33.75 mg GAE/g extract, 16.17 mg QE/g extract, 762.58 mg TE/g extract, and 1221.57 mg TE/g extract, respectively. Combination ratios of 1:1, 1:2, 2:1, 1:3, and 3:1 tended to show additive effects. Lipase inhibition by single secang (70.96%) was better than all combinations, but not better than orlistat (76.83%).