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Title: | Analisis Sifat Antikanker dan Profil Metabolit Sekunder Ekstrak Dadih Komersial dengan Lama Fermentasi Berbeda |
Other Titles: | Analysis of Anticancer Properties and Secondary Metabolite Profile of Commercial Dadih Extracts with Different Fermentation Durations |
Authors: | Budiman, Cahyo Arief, Irma Isnafia Fadhila, Luthfia Widyasari |
Issue Date: | 2024 |
Publisher: | IPB University |
Abstract: | Dadih merupakan makanan tradisional asal Minangkabau yang dibuat dari
fermentasi susu kerbau melalui penyimpanan suhu ruang di dalam tabung bambu.
Susu kerbau mengandung peptida bioaktif yang bersifat antimikroba,
imunomodulator, antikanker, antidiabetik, antihipertensi, dan antioksidan.
Penelitian ini bertujuan menganalisis sifat antikanker dan profil metabolit sekunder
dadih komersial. Metode MTT Assay digunakan untuk mengetahui tingkat
toksisitas senyawa ekstrak dadih (5%, 10%, 20%, dan 40%) terhadap pertumbuhan
sel MCF-7. Hasil pengujian menunjukkan bahwa sampel A1, E1, A7, dan E7
memiliki nilai IC50 = 4,7-17 µg mL-1 dengan tingkat aktivitas antikanker yang kuat.
Profil metabolit sekunder dianalisis menggunakan Liquid chromatography-mass
spectrometry dan metode PCA, dengan nilai eigenvalue dan eigenvectors dari dua
komponen utama minimal 84,2%. Beberapa senyawa metabolit sekunder ekstrak
dadih memiliki peran fungsional terhadap akivitas biologis serta farmakologis,
salah satunya antikanker. Dadih is a traditional Minangkabau cuisine made from fermented buffalo milk, stored at room temperature in bamboo tubes. Buffalo milk contains bioactive peptides with antimicrobial, immunomodulatory, anticancer, antidiabetic, antihypertensive, and antioxidant properties. This study aims to analyze the anticancer properties and secondary metabolite profile of commercial dadih. The MTT Assay method was used to determine the toxicity levels of dadih extract compounds (5%, 10%, 20%, and 40%) on the growth of MCF-7 cells. The test results showed that samples A1, E1, A7, and E7 had IC50 = 4,7-17 µg mL-1 , indicating strong anticancer activity. The secondary metabolite profile was analyzed using Liquid chromatography-mass spectrometry and PCA methods, with the eigenvalues and eigenvectors of the two main components at least 84.2%. Several secondary metabolite compounds in dadih extract have functional roles in biological and pharmacological activities, one of which is anticancer. |
URI: | http://repository.ipb.ac.id/handle/123456789/154825 |
Appears in Collections: | UT - Technology of Cattle Products |
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File | Description | Size | Format | |
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cover_D3401201001_4f3fa932bf4c4dcaba2d31598f0e0f9f.pdf | Cover | 349.92 kB | Adobe PDF | View/Open |
fulltext_D3401201001_38d0d17912de47d6b601ec41137ef7fa.pdf Restricted Access | Fulltext | 1.39 MB | Adobe PDF | View/Open |
lampiran_D3401201001_2f415050ab794504aecc6119bb889db3.pdf Restricted Access | Lampiran | 347.48 kB | Adobe PDF | View/Open |
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