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Title: | Aktivitas Inhibisi Lipase Pankreas dan Antioksidan Daun Kelor (Moringa oleifera) sebagai Antiobesitas in Vitro |
Other Titles: | Pancreatic Lipase Inhibition and Antioxidant Activity of Kelor Leaf (Moringa oleifera) as Antiobesity in Vitro |
Authors: | Hasim, Hasim Faridah, Didah Nur Ramayani, Desty |
Issue Date: | 2024 |
Publisher: | IPB University |
Abstract: | Daun kelor berpotensi sebagai antiobesitas, tetapi belum diketahui metabolit
yang terlibat sehingga identifikasi senyawa diperlukan. Penelitian ini bertujuan
menentukan fraksi terbaik, menganalisis fitokimia, aktivitas antioksidan dan
inhibisi enzim lipase, serta membandingkan korelasi antara semua perlakuan. Daun
kelor disonikasi dengan etanol 70%, lalu difraksinasi bertingkat menggunakan n heksana, kloroform, etil asetat, dan akuades. Total phenolic content (TPC) dan total
flavonoid content (TFC) diukur secara kolorimetri dengan Folin-ciocalteu dan
alumunium klorida. Aktivitas antioksidan diukur menggunakan metode DPPH dan
FRAP. Inhibisi lipase pankreas diukur secara enzimatis dengan p-nitrophenyl
glucopyranoside. Hasil TPC, TFC, DPPH, dan FRAP tertinggi berturut-turut
dimiliki oleh ekstrak akuades (138,88 ± 4,42 mg GAE/g ekstrak), etil asetat (113,31
± 2,02 mg QE/g ekstrak), etanol 70% (112,49 ± 0,33 mg GAE/g ekstrak), dan
akuades (1263,57 ± 0,04 mg TE/g ekstrak). Nilai IC50 paling rendah yaitu ekstrak
akuades 545,87 ppm. Dengan demikian, ekstrak akuades berpotensi untuk
dikembangkan sebagai obat herbal antiobesitas. Kelor leaves have potential as an anti-obesity agent, but the metabolites aren’t yet known so compound identification is necessary. This research aimed to determine the best fraction, analyze phytochemicals, antioxidant and lipase enzyme inhibition activity, and compare the correlation between all treatments. Kelor leaves were sonicated with 70% ethanol and then fractionated using n-hexane, chloroform, ethyl acetate, and distilled water. Total phenolic content (TPC) and total flavonoid content (TFC) were measured colorimetrically with Folin-ciocalteu and aluminum chloride. Antioxidant activity was measured using the DPPH and FRAP methods. Pancreatic lipase inhibition was measured enzymatically with the p-nitrophenyl glucopyranoside. The highest TPC, TFC, DPPH, and FRAP results were respectively obtained by distilled water (138.88 ± 4.42 mg GAE/g extract), ethyl acetate (113.31 ± 2.02 mg QE/g extract), ethanol 70% (112.49 ± 0.33 mg GAE/g extract), and distilled water (1263.57 ± 0.04 mg TE/g extract). The lowest IC50 value was distilled water 545.87 ppm. Thus, distilled water extract has the potential to be developed as an anti-obesity herbal medicine. |
URI: | http://repository.ipb.ac.id/handle/123456789/153147 |
Appears in Collections: | UT - Biochemistry |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover.pdf Restricted Access | Cover | 1.26 MB | Adobe PDF | View/Open |
G8401201024_Desty Ramayani.pdf Restricted Access | Fulltext | 1.92 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 632.53 kB | Adobe PDF | View/Open |
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