Aktivitas Inhibisi Lipase Pankreas dan Antioksidan Daun Kelor (Moringa oleifera) sebagai Antiobesitas in Vitro

Abstract

Daun kelor berpotensi sebagai antiobesitas, tetapi belum diketahui metabolit yang terlibat sehingga identifikasi senyawa diperlukan. Penelitian ini bertujuan menentukan fraksi terbaik, menganalisis fitokimia, aktivitas antioksidan dan inhibisi enzim lipase, serta membandingkan korelasi antara semua perlakuan. Daun kelor disonikasi dengan etanol 70%, lalu difraksinasi bertingkat menggunakan n heksana, kloroform, etil asetat, dan akuades. Total phenolic content (TPC) dan total flavonoid content (TFC) diukur secara kolorimetri dengan Folin-ciocalteu dan alumunium klorida. Aktivitas antioksidan diukur menggunakan metode DPPH dan FRAP. Inhibisi lipase pankreas diukur secara enzimatis dengan p-nitrophenyl glucopyranoside. Hasil TPC, TFC, DPPH, dan FRAP tertinggi berturut-turut dimiliki oleh ekstrak akuades (138,88 ± 4,42 mg GAE/g ekstrak), etil asetat (113,31 ± 2,02 mg QE/g ekstrak), etanol 70% (112,49 ± 0,33 mg GAE/g ekstrak), dan akuades (1263,57 ± 0,04 mg TE/g ekstrak). Nilai IC50 paling rendah yaitu ekstrak akuades 545,87 ppm. Dengan demikian, ekstrak akuades berpotensi untuk dikembangkan sebagai obat herbal antiobesitas.

Kelor leaves have potential as an anti-obesity agent, but the metabolites aren’t yet known so compound identification is necessary. This research aimed to determine the best fraction, analyze phytochemicals, antioxidant and lipase enzyme inhibition activity, and compare the correlation between all treatments. Kelor leaves were sonicated with 70% ethanol and then fractionated using n-hexane, chloroform, ethyl acetate, and distilled water. Total phenolic content (TPC) and total flavonoid content (TFC) were measured colorimetrically with Folin-ciocalteu and aluminum chloride. Antioxidant activity was measured using the DPPH and FRAP methods. Pancreatic lipase inhibition was measured enzymatically with the p-nitrophenyl glucopyranoside. The highest TPC, TFC, DPPH, and FRAP results were respectively obtained by distilled water (138.88 ± 4.42 mg GAE/g extract), ethyl acetate (113.31 ± 2.02 mg QE/g extract), ethanol 70% (112.49 ± 0.33 mg GAE/g extract), and distilled water (1263.57 ± 0.04 mg TE/g extract). The lowest IC50 value was distilled water 545.87 ppm. Thus, distilled water extract has the potential to be developed as an anti-obesity herbal medicine.