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Title: | Identification and Nucleotide Sequence Variation of Begomovirus Causing Yellow Mosaic Disease on Several Crops |
Other Titles: | Identifikasi dan Variasi Sekuen Nukleotida Begomovirus Penyebab Penyakit Mosaik Kuning pada Beberapa Tanaman |
Authors: | Hidayat, Sri Hendrastuti Nurulita, Sari Hanifa, Ledia |
Issue Date: | 20-Dec-2023 |
Publisher: | IPB University |
Abstract: | Begomovirus is one of genera in the family Geminiviridae that has been
reported globally to cause significant yield losses on crops production. Its infection
causess typical symptoms, such as curling, yellow mosaic, and yellowing on the
leaf. In Indonesia, yellow mosaic diseases associated with various Begomoviruses
have been reported on chili pepper, yard long bean, cucumber and eggplant.
Therefore, the aims of this study are (i) to detect Begomoviruses infecting chili
pepper, yard long bean, cucumber, and eggplant and (ii) to analyze their genetic
variation to determine the dynamics of Begomovirus development in Indonesia in
the last ten years. Leaf samples were collected from infected plants (chili pepper,
yard long bean, cucumber, and eggplant) in Bogor (West Java) and Brebes (Central
Java). In addition, Begomovirus nucleic acid from the collection of Plant Virology
Laboratory were used as comparison. These samples were isolated ten years ago
from Bogor (West Java) and Magelang, Klaten, and Brebes (Central Java).
Begomovirus detection was carried out by polymerase chain reaction (PCR)
method using a universal primer pair (SPG1/SPG2) followed by nucleotide
sequence analysis. DNA samples were successfully amplified from both field and
laboratory samples. Three Begomovirus species were identified, i.e., Pepper yellow
leaf curl Indonesia virus (PYLCIV) on chili pepper, Mungbean yellow mosaic India
virus (MYMIV) on yard long bean and Tomato leaf curl New Delhi virus
(ToLCNDV) on cucumber and eggplant. Phylogenetic tree analysis showed that all
samples from the field and laboratory were grouped in one cluster. However,
nucleotide base differences were found in each Begomovirus species. This finding
indicates a change in the nucleotide sequence of the virus over a period of time. Begomovirus adalah salah satu genera dalam famili Geminiviridae yang telah dilaporkan secara global dapat menyebabkan kehilangan hasil yang nyata pada produksi tanaman. Gejala infeksi Begomovirus umumnya menimbulkan gejala khas, seperti keriting, mosaik kuning, dan daun menguning. Di Indonesia, penyakit mosaik kuning yang diasosiasikan dengan berbagai Begomovirus telah dilaporkan terjadi pada tanaman cabai, kacang panjang, mentimun, dan terung. Oleh karena itu, penelitian ini bertujuan untuk (i) mendeteksi Begomovirus yang menginfeksi tanaman cabai, kacang panjang, mentimun, dan terung, dan (ii) menganalisis variasi genetiknya untuk mengetahui dinamika perkembangan Begomovirus di Indonesia dalam sepuluh tahun terakhir. Sampel daun dikumpulkan dari tanaman yang terinfeksi (cabai, kacang panjang, mentimun, dan terung) di Bogor (Jawa Barat) dan Brebes (Jawa Tengah). Selain itu, sampel asam nukleat Begomovirus dari koleksi Laboratorium Virologi Tumbuhan digunakan sebagai pembanding. Sampel tersebut diisolasi sepuluh tahun yang lalu dari tanaman cabai, kacang panjang, mentimun, dan terung dari beberapa lokasi. Deteksi Begomovirus dilakukan dengan metode polymerase chain reaction (PCR) menggunakan pasangan primer universal (SPG1/SPG2) yang dilanjutkan dengan analisis sekuen nukleotida. Sampel DNA berhasil diamplifikasi dari sampel lapangan dan laboratorium. Tiga spesies Begomovirus berhasil diidentifikasi, yaitu Pepper yellow leaf curl Indonesia virus (PYLCIV) pada cabai, Mungbean yellow mosaic India virus (MYMIV) pada kacang panjang, dan Tomato leaf curl New Delhi virus (ToLCNDV) pada mentimun dan terung. Analisis pohon filogenetik menunjukkan bahwa semua sampel dari lapangan dan laboratorium mengelompok dalam satu klaster. Namun, perbedaan basa nukleotida ditemukan pada setiap spesies Begomovirus. Temuan ini mengindikasikan adanya perubahan sikuen nukleotida virus dalam kurun waktu tertentu. |
URI: | http://repository.ipb.ac.id/handle/123456789/133297 |
Appears in Collections: | UT - Plant Protection |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover.pdf Restricted Access | Cover | 2.67 MB | Adobe PDF | View/Open |
A34190044_Ledia Hanifa 3.pdf Restricted Access | Fullteks | 5 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Attachment | 4.38 MB | Adobe PDF | View/Open |
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