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Title: | Penghambatan Autooksidasi Lipid oleh Ekstrak Maggot Black Soldier Fly (Hermetia illucens) |
Authors: | Sulistiyani Purwanto, Ukhradiya Magharaniq Safira Sujai, Puji Ayu Ningtyas |
Issue Date: | 2023 |
Publisher: | IPB University |
Abstract: | Stres oksidatif merupakan kondisi ketidakseimbangan antara produksi dan degradasi senyawa radikal bebas di dalam tubuh. Jumlah radikal bebas berlebih dapat diatasi dengan adanya antioksidan. Maggot Black Soldier Fly diketahui dapat mengaktifkan persinyalan antioksidan tubuh sehingga berpotensi dijadikan sumber antioksidan alami. Penelitian ini bertujuan mengidentifikasi metabolit dan menganalisis penghambatan proses autooksidasi lipid oleh ekstrak kasar maggot Black Soldier Fly asal Desa Sindangjaya, Cianjur. Sebanyak total 83 senyawa diidentifikasi pada ekstrak kasar maggot berdasarkan analisis menggunakan Liquid Chromatography Tandem-Mass Spectrometry. Aktivitas antioksidan tertinggi berdasarkan metode thiobarbituric acid dihasilkan oleh ekstrak metanol maggot yang diberi pakan limbah dapur, dengan nilai inhibisi sebesar 94,71% pada konsentrasi 5 μg/mL. Nilai ini jauh lebih tinggi dibandingkan dengan α-tokoferol yang hanya menghasilkan nilai inhibisi sebesar 74,85% pada konsentrasi yang sama. Ekstrak maggot yang diberi pakan limbah dapur memiliki aktivitas antioksidan yang lebih kuat dibandingkan dengan yang diberi pakan limbah pertanian. Oxidative stress is a condition of imbalance between the production and degradation of free radicals in the body. Excessive amounts of free radicals can be counteracted by the presence of antioxidants. The Black Soldier Fly maggot is known to activate antioxidant signaling, making it a potential source of natural antioxidants. This research aimed to identify metabolites and analyze the inhibition of lipid autooxidation process by crude extract of Black Soldier Fly maggot from Sindangjaya Village, Cianjur. A total of 83 compounds were identified in maggot crude extract based on Liquid Chromatography Tandem-Mass Spectrometry analysis. The highest antioxidant activity based on the thiobarbituric acid method was produced by the methanol extract of maggot fed with kitchen waste, with an inhibition value of 94.71% at a concentration of 5 μg/mL. This value was much higher than α-tocopherol which only produced an inhibition value of 74.85% at the same concentration. Maggot extracts fed with kitchen waste had stronger antioxidant activity than those fed with agricultural waste. |
URI: | http://repository.ipb.ac.id/handle/123456789/129627 |
Appears in Collections: | UT - Biochemistry |
Files in This Item:
File | Description | Size | Format | |
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Cover.pdf Restricted Access | Cover | 538.94 kB | Adobe PDF | View/Open |
G84190032_Puji Ayu Ningtyas Sujai.pdf Restricted Access | Fulltext | 878.09 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 840.39 kB | Adobe PDF | View/Open |
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