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http://repository.ipb.ac.id/handle/123456789/125821
Title: | Virgin Deep Sea Fish Oil Lepidocybium flavobrunneum dan Ruvettus pretiosus serta Potensinya sebagai Imunostimulan |
Authors: | Suseno, Sugeng Heri Ramadhan, Wahyu Sari, Gita Novita |
Issue Date: | 2023 |
Publisher: | IPB University |
Abstract: | Ikan gindara dan gindara kulit duri merupakan ikan hasil tangkapan
sampingan (by catch) dari ikan tuna yang dapat hidup pada kedalaman 200 meter
dibawah permukaan air laut. Penelitian ini bertujuan menentukan nilai proksimat,
rendemen minyak, profil asam lemak, parameter oksidasi dan potensi minyak ikan
laut dalam sebagai agen imunostimulan secara in vitro. Ekstraksi minyak ikan laut
dalam dilakukan dengan metode wet rendering suhu rendah (40, 50, 60, 70 ℃) dan
pengujian proliferasi sel splenosit dengan uji MTT assay. Asam lemak yang
dominan pada ikan gindara dan gindara kulit duri adalah asam oleat (C18:1n-9c)
dengan nilai 26,85% (b/b) ikan gindara dan 23,02% (b/b) ikan gindara kulit duri.
Minyak ikan gindara dan gindara kulit duri mengandung asam oleat yang tinggi.
Hasil pengujian aktivitas proliferasi sel splenosit menunjukkan minyak ikan gindara
dan gindara kulit duri suhu ekstraksi 70℃ dapat meningkatkan proliferasi sel
splenosit sehingga berpotensi sebagai imunostimulan. Gindara and thorn skin gindara are by-catch fish from tuna which can live at a depth of 200 meters below sea level. This study aims to determine the proximate value, oil yield, fatty acid profile, oxidation parameters and the potential of deep sea fish oil as an immunostimulating agent in vitro. Extraction of deep sea fish oil was carried out using the low temperature wet rendering method (40,50,60,70℃) and splenocyte cell proliferation testing with the MTT assay test. The dominant fatty acid in gindara fish and thorn skin gindara was oleic acid (C18:1n-9c) with a value of 26.85% (w/w) for gindara fish and 23.02% (w/w) for gindara thorn fish. Gindara fish oil and gindara thorn skin contained high oleic acid. The results of testing the proliferative activity of splenocyte cells showed that gindara fish oil and gindara spines at an extraction temperature of 70℃ could increase splenocyte cell proliferation thus that they had the potential as immunostimulants. |
URI: | http://repository.ipb.ac.id/handle/123456789/125821 |
Appears in Collections: | UT - Aquatic Product Technology |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
01 Watermark (Cover) - SKRIPSI - (C34180018) GITA NOVITA SARI.pdf Restricted Access | Cover | 256.33 kB | Adobe PDF | View/Open |
02 Watermark (Fullteks) - SKRIPSI - (C34180018) GITA NOVITA SARI.pdf Restricted Access | Fullteks | 627.35 kB | Adobe PDF | View/Open |
03 Watermark (Lampiran) - SKRIPSI - (C34180018) GITA NOVITA SARI.pdf Restricted Access | Lampiran | 363.8 kB | Adobe PDF | View/Open |
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