Optimasi Proses Esktraksi Gelatin Kulit Patin (Pangasius sp.) dengan Aplikasi Response Surface Methodology

Abstract

Ikan patin merupakan bahan baku gelatin alternatif yang aman dan halal. Kulit ikan memiliki karakteristik yang berbeda dengan hewan terestrial sehingga memerlukan optimasi pada proses ekstraksi sebelum masuk ke skala industri. Penelitian ini bertujuan melakukan optimasi kondisi pretreatment pada proses ekstraksi gelatin menggunakan Response Surface Methodology (RSM) serta menentukan karakteristik gelatin terpilih. Rancangan eksperimen yang digunakan yaitu Central Composit Design (CCD) dengan dua variabel bebas (konsentrasi NaOH dan konsentrasi asam asetat) dan 7 variabel respon (rendemen, kadar air, kadar abu, kekuatan gel, viskositas, pH, dan setting point). Hasil optimasi diperoleh respon optimum pada konsentrasi NaOH 0,3 M dan konsentrasi asam asetat 0,067 M. Hasil validasi keadaan optimal memperoleh gelatin terpilih dengan rendemen 22,44±0,68%, kadar air 8,53±3,4%, kadar abu 0,325±0,22%, derajat keasaman (pH) 6,65±0,13, kekuatan gel 241,58±9,5 Bloom, viskositas 6,65±0,5 mPas, dan setting point 19,75±1,06 ℃. Karakteristik gelatin terpilih telah memenuhi standar gelatin SNI 8622:2018 dan GMIA 2019.

Catfish is an alternative raw material for gelatin that is halal and safe. Pangasius skin has different characteristics from terrestrial animals, so it requires to optimize the extraction process before industrial scale production. This study aims to optimize the pretreatment conditions in the gelatin extraction process using the Response Surface Methodology (RSM) and determine the characteristics of the selected gelatin based on the pretreatment conditions. Central Composite Design (CCD) with two independent variables (NaOH concentration and acetic acid concentration) and 7 response variables (yield, moisture content, ash content, gel strength, viscosity, pH, and setting point) was performed. The results showed the optimum response at 0.3 M NaOH and 0.067 M acetic acid. The validation obtained selected gelatin with a yield 22.44 ± 0.68%, water content 8.53 ± 3.4%, ash content 00.325 ± 0.22%, degree of acidity (pH) 6.65 ± 0.13, gel strength 241.58 ± 9.5 Bloom, viscosity 6.65 ± 0.45 mPas, and setting point 19.75 ± 1.06 ℃. The characteristics of the selected gelatin have met the SNI 8622: 2018 and GMIA 2019 gelatin standards.