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Title: | Pengaruh Media dan Pembelahan Embrio pada Kultur Embrio Tanaman Pinang (Areca catechu L.) secara In Vitro |
Other Titles: | Effects of Media and Embryo Division on In Vitro Embryo culture of Areca Nut (Areca catechu L.) |
Authors: | Dinarti, Diny Amarillis, Shandra Siregar, Fezilo Rivansyah |
Issue Date: | 2022 |
Publisher: | IPB University |
Abstract: | Pinang (Areca catechu L.) merupakan salah satu komoditas yang
mengalami peningkatan ekspor setiap tahunnya. Perbanyakan pinang hingga saat
ini masih terkendala penyediaan bibit. Aplikasi kultur embrio dengan pembelahan
merupakan salah satu upaya untuk penyediaan bibit. Penelitian ini bertujuan
mempelajari pengaruh media dan pembelahan embrio pada kultur embrio pinang
secara in vitro. Penelitian menggunakan rancangan kelompok lengkap teracak
(RKLT) faktorial dengan 2 faktor. Faktor pertama yaitu jenis media yang
didasarkan pada 3 penelitian terdahulu. Faktor lainnya yaitu teknik pembelahan
yang terdiri atas 3 taraf (tanpa dibelah, dilukai menjadi dua, dibelah menjadi dua).
Terdapat 9 perlakuan diulang sebanyak 3 kali sehingga ada 27 satuan percobaan
dan setiap satuan percobaan terdiri atas 4 botol kultur yang masing-masing ditanam
satu embrio, seluruhnya terdapat 108 satuan amatan. Media dasar yang digunakan
adalah Murashige dan Skoog yang ditambahkan air kelapa 15% dan arang aktif 2 g
L-1. Analisis data menggunakan uji F dan uji lanjut BNJ. Media berpengaruh
terhadap persentase eksplan hidup pada minggu ke 8. Komposisi BAP 1 ppm dan
NAA 0,1 ppm sama baiknya dengan media yang mengandung 2ip 4,1 ppm terhadap
persentase eksplan hidup. Perlakuan pembelahan menunjukkan pengaruh nyata
terhadap persentase kematian dan browning eksplan. Eksplan tanpa pembelahan
menghasilkan persentase browning nyata lebih sedikit dibanding perlakuan
pelukaan menjadi dua dan pembelahan menjadi dua. Terdapat interaksi pada
minggu 4 dan 12 terhadap eksplan hidup. Belum diperoleh komposisi zat pengatur
tumbuh terbaik dan cara pembelahan embrio untuk meningkatkan tunas in vitro
pinang. Areca nut (Areca catechu L.) is one of the commodities that has increased exports every year. The propagation of areca nut is still constrained by the provision of seedlings. Application of embryo culture by division is one of the efforts to provide seedlings. This study aimed to study the effect of media and embryo division on areca nut embryo culture in vitro. The study used a factorial randomized complete block design (RKLT) with 2 factors. The first factor was the type of media based on 3 previous studies. Another factor was the cleavage technique which consists of 3 levels (without being split, incision in half, split into two). There were 9 treatments repeated 3 times so that there were 27 experimental units and each experimental unit consisted of 4 culture bottles, each of which was planted with one embryo, there were 108 units of observation in total. The basic media used was Murashige and Skoog which were added with 15% coconut water and 2 g L-1 activated charcoal. Data analysis used F test and BNJ follow-up test. The media affected the percentage of lived explants at week 8. The composition of 1 ppm BAP and 0.1 ppm NAA was as good as the media containing 2ip 4.1 ppm on the percentage of live explants. The splitting treatment showed a significant effect on the percentage of mortality and browning of explants. Explants without cleavage resulted in significantly less browning percentage than the treatment of incision in half and splitting into two. There was an interaction at weeks 4 and 12 with live explants. The composition of the best plant growth regulators and methods of embryo division to increase shoots in vitro have not yet been obtained. |
URI: | http://repository.ipb.ac.id/handle/123456789/114110 |
Appears in Collections: | UT - Agronomy and Horticulture |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
COVER_FEZILO RIVANSYAH SIREGAR (A24180049).pdf Restricted Access | Cover | 426.32 kB | Adobe PDF | View/Open |
A24180049_Fezilo Rivansyah Siregar.pdf Restricted Access | Fullteks | 873.97 kB | Adobe PDF | View/Open |
LAMPIRAN_FEZILO RIVANSYAH SIREGAR (A24180049).pdf Restricted Access | Lampiran | 176.66 kB | Adobe PDF | View/Open |
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