Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/113751
Title: | Ekstraksi Minyak Ikan dari Jeroan Ikan Air Laut serta Potensinya sebagai Imunostimulan |
Authors: | Suseno, Sugeng Heri Nugraha, Roni Insani, Azzellya Permata |
Issue Date: | 2022 |
Publisher: | IPB University |
Abstract: | Minyak ikan diketahui memiliki kandungan omega-3 yang tinggi dan baik
untuk kesehatan. Kandungan poly unsaturated fatty acids (PUFA) seperti omega-3
pada minyak ikan memiliki efek imunomodulator yang potensial. Penelitian ini
bertujuan untuk mengetahui profil asam lemak serta potensi minyak ikan dari jeroan
ikan air laut sebagai imunostimulan. Minyak ikan diekstraksi dari 7 jenis jeroan
ikan air laut dengan metode bligh and dyer. Pengujian aktivitas imunostimulan
dilakukan dengan melihat proliferasi sel splenosit mencit menggunakan metode
MTT assay. Asam lemak dominan pada jeroan ikan bawal hitam, kakap merah,
makarel, kembung dan selar kuning adalah asam palmitat, sedangkan asam lemak
dominan pada jeroan ikan tongkol abu dan mahi-mahi adalah docosahexaenoic acid
(DHA). Jeroan ikan makarel dan tongkol abu mengandung PUFA tertinggi
sehingga digunakan pada pengujian aktivitas proliferasi sel splenosit. Hasil
pengujian aktivitas proliferasi sel splenosit menunjukkan ekstrak kasar minyak
jeroan ikan makarel dan tongkol abu dapat meningkatkan proliferasi sel splenosit
sehingga berpotensi sebagai imunostimulan. Fish oil is known to have high omega-3 content and also good for health. The content of poly unsaturated fatty acids (PUFA) such as omega-3 in fish oil has a potential immunomodulatory effect. This study aims to determine the fatty acid profile and the potential of fish oil from the viscera of seawater fishes as an immunostimulant. Fish oil was extracted from 7 types of seawater fish viscera using the bligh and dyer method. Testing of immunostimulant activity was carried out by observing the proliferation of splenocyte cells of mice using the MTT assay method. The dominant fatty acid in black pomfret, red snapper, chub mackerel, indian mackerel and yellowstripe scad visceras were palmitic acid, while the dominant fatty acid in longtail tuna and dolphinfish visceras were docosahexaenoic acid (DHA). Chub mackerel and longtail tuna fish oil contained the highest PUFA, so they were used in testing the proliferative activity of splenocytes. The results of the splenocyte cell proliferation activity test showed that crude extract of chub mackerel and longtail tuna viscera fish oil increased the proliferation of splenocyte cells so that they have potential as immunostimulants. |
URI: | http://repository.ipb.ac.id/handle/123456789/113751 |
Appears in Collections: | UT - Aquatic Product Technology |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
COVER_AZZELLYA PERMATA INSANI (C34170055).pdf Restricted Access | Cover | 520.4 kB | Adobe PDF | View/Open |
FULL TEXT_AZZELLYA PERMATA INSANI (C34170055).pdf Restricted Access | Full Text | 1.1 MB | Adobe PDF | View/Open |
LAMPIRAN_AZZELLYA PERMATA INSANI (C34170055).pdf Restricted Access | Lampiran | 741.49 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.