| dc.description.abstract | Toxicity effects of Gynura pseudochina (Linn). DC extracted with various solvents were assessed in vitro. The extracted compounds were divided into two major groups, semi polar and polar compounds and were tested for toxicity using {HeLa (ATTC CCL-2), Hep2 (ATTC CCL-23), and Raji (ATCC CCL-86)) cell lines. Fractionation was performed by thin layer chromatography and follow by column chromatography using silica gel as the adsorbent. Six fractions containing semi polar compound were obtained using chloroform: methanol (7:l) as the eluent. Four polar compounds were obtained using methanol: acetic acid (8:2) as the eluent. The fraction of semi polar compounds tested in HeLa, Hep-2, and Raji cell lines at 250 ppm showed that the highest inhibition are fraction VI (42.4%) in HeLa cells, fraction IllNl (24.1%) in Hep-2 cells, and fraction IV (25.2%) in Raji cells. The highest inhibition of polar compounds at 250 ppm are fraction II in Raji cells (46.6%), fraction Ill in HeLa cells (22.7%), and fraction IV in Hep-2 cells (41.7%). At 500 ppm, all polar compounds showed an increased inhibition in Raji cells (55.4% - 97%) and HeLa cells (21.3%-26.7%), but decreased at Hep-2 cell (-1.3%-8.35%). Characterization of polar compounds by qualitative phytochemistry assay showed the presence of steroid group. Spectroscopy data from UV and IR spectrums showed that hmax of the compounds is 212-220 nm and consist of -OH and C=C groups | id |
